Test Catalog

Test ID: VWD8B    
von Willebrand Disease 2N (Subtype Normandy), Plasma

Method Description Describes how the test is performed and provides a method-specific reference

The von Willebrand Factor (VWF):Factor VIII B (FVIIIB) assay utilizes enzyme linked immunosorbent assay (ELISA) technology. A diluted plasma sample adjusted to 10 IU dL of VWF:Antigen (Ag) is incubated with a rabbit antihuman VWF F(ab’)2, which is coated on the internal walls of the microplate wells. The factor VIII of the tested plasma dissociated during the incubation is washed away. Recombinant FVIII is then added, which binds to VWF. Next, mouse monoclonal anti-human FVIII antibody coupled with peroxidase binds to the remaining free antigenic determinants of the bound FVIIIr. Bound FVIIIr is quantified using a peroxidase-conjugated mouse antihuman FVIII monoclonal antibody. The intensity of the color is directly proportional with the concentration of VWF:FVIIIB initially present in the plasma sample.(Package Insert: Asserachrom VWF:FVIIIB. Diagnostica Stago, Parsippany, NJ, Revised March 2014)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.


Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

1 to 10 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test