TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: NPM1Q    
Nucleophosmin (NPM1) Mutation Analysis, Varies

Method Description Describes how the test is performed and provides a method-specific reference

RNA is extracted from blood or bone marrow and reverse transcription is performed. Real time quantitative polymerase chain reaction (PCR) is performed from complementary DNA (cDNA) template using the LC480 instrument platform (Roche). This assay targets the most common recurrent nucleophosmin (NPM1) alterations in acute myeloid leukemia (AML) (A, B, and D insertion variants). The quantitative value of NPM1 mRNA copy number is determined relative to ABL1 as the reference transcript using the delta-delta CT method. The reproducible analytical sensitivity (limit of detection) of this part of the assay is approximately 0.01%.

 

DNA is extracted from blood or bone marrow and a PCR assay is performed using primers that amplify a fragment of NPM1 DNA containing the region susceptible to insertion variant. One of the PCR primers contains a fluorescent label. The amplified fragments are size separated by capillary electrophoresis. Wild type NPM1 produces a fragment length of 187-base pairs (bp). PCR fragments containing an insertional variant are observed as larger fragments, most typically 191-bp, as the majority of alterations are 4-bp insertions. The analytical sensitivity (limit of detection) of this part of the assay is approximately 5%.(Unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

Supplemental

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

10 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

14 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

RNA and DNA 3 months

Performing Laboratory Location Indicates the location of the laboratory that performs the test

Rochester