Test Catalog

Test ID: VWFMP    
von Willebrand Factor Multimer Analysis, Plasma

Method Description Describes how the test is performed and provides a method-specific reference

Platelet-poor plasma proteins are denatured using heat and an anionic detergent, sodium dodecyl sulfate. The sample is then electrophoresed through a discontinuous agarose gel on a cooled horizontal electrophoresis unit overnight to separate the von Willebrand factor (VWF) multimers by size. The gel is fixed in acid and isopropanol, washed in water, and incubated with dilute rabbit antihuman VWF. After washing away unbound antibody, the gel is incubated with dilute goat antirabbit IgG antibody tagged with an infrared dye. Excess secondary antibody is washed away, and the gel is scanned using an infrared imaging system. The digitized image of the electrophoretic distribution of the VWF multimers is interpreted by a coagulation consultant and a written report is provided.(Ruggeri ZM, Zimmerman TS: Variant von Willebrand's disease. Characterization of two subtypes by analysis of multimeric composition of FVIII/vWF in plasma and platelets. J Clin Invest 1980;65:1318-1325)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday, Tuesday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

7 to 14 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

21 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test