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Test Catalog

Test ID: HIVDX    
HIV-1 and HIV-2 Antigen and Antibody Diagnostic Evaluation, Plasma

Method Description Describes how the test is performed and provides a method-specific reference

The Abbott Architect HIV Ag/Ab Combo assay for use on the Architect i System is a 2-step immunoassay to determine the presence of HIV-1 p24 antigen, antibodies to HIV-1 (groups M and O), and antibodies to HIV-2 in human serum or plasma using chemiluminescent microparticle immunoassay technology. First, patient's specimen, Architect i wash buffer, assay diluent, and paramagnetic microparticles are combined in a single reaction well. HIV-1 p24 antigen and HIV-1/HIV-2 antibodies present in the patient's specimen bind to the HIV-1 antigen-, HIV-2 antigen-, and HIV-1 p24 monoclonal (mouse) antibody-coated microparticles. After washing, the bound HIV-1 p24 antigen and HIV-1/HIV-2 antibodies bind to the acridinium-labeled conjugates. Following another wash cycle, pretrigger and trigger solutions are added to the reaction mixture. The resulting chemiluminescent reaction is measured as relative light units (RLU). The amount of HIV antigen and antibodies present in the patient's specimen is directly proportionate to the RLU detected by the Architect i System optics. The presence or absence of HIV-1 p24 antigen or HIV-1/HIV-2 antibodies in the specimen is determined by comparing the chemiluminescent signal in the reaction to the cutoff signal determined periodically by assay calibration. Patients' specimens with signal-to-cutoff (S/CO) values greater than or equal to 1.00 are considered reactive for HIV-1 p24 antigen or HIV-1/HIV-2 antibodies. Specimens with S/CO values under 1.00 are considered nonreactive or negative.(Package insert: HIV Ag/Ab Combo. Abbott Laboratories; 08/2017)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Saturday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

2 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test

Rochester