Assisting in the diagnostic process of ankylosing spondylitis,
Flow Cytometry
B27
Histocompatibility Antigen
Human Leukocyte Antigen
Leukocyte Antigens
LY27
Spondylitis
W-27 (HLA-B27)
Whole Blood EDTA
This test is best used alone if a particular disease such as ankylosing spondylitis is under consideration.
Orders received for both this test and 1LRR / Human Leukocyte Antigens (HLA) Class I Typing Low Resolution, Recipient, Blood or 1DIS / Human Leukocyte Antigens (HLA) A-B-C Disease Association Typing Low Resolution, Blood (which provides data on all HLA Class I low-resolution antigens, including B27) will be questioned due to test overlap.
Specimen must arrive within 96 hours of collection.
Container/Tube: Lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
1 mL
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole Blood EDTA | Ambient (preferred) | 4 days |
Assisting in the diagnostic process of ankylosing spondylitis,
This major histocompatibility coded class I antigen is associated with ankylosing spondylitis, juvenile rheumatoid arthritis, and Reiter syndrome. The mechanism of the association is not understood but probably is that of linkage disequilibrium.
There is an increased prevalence of HLA-B27 in certain rheumatic diseases, particularly ankylosing spondylitis.
Studies have demonstrated that the B*27:06 allele, which is present in a small percentage of individuals of Asian ethnicity, may not be associated with ankylosing spondylitis.
An interpretive report will be provided.
Approximately 8% of the normal population carries the HLA-B27 antigen.
HLA-B27 is present in approximately 89% of patients with ankylosing
Extreme temperature changes during shipping may alter the specimen making it unacceptable for testing.
1. Profaizer T, Dibb K, Bethers H, et al: Comparison of next-generation sequencing-based human leukocyte antigen typing with clinical flow cytometry and allele-specific PCR melting assays for HLA-B27 genotyping. J Appl Lab Med. 2021 Sep 1;6(5):1221-1227. doi: 10.1093/jalm/jfab046
2. Skalska U, Kozakiewicz A, Maslinski W, Jurkowska M: HLA-B27 detection - comparison of genetic sequence-based method and flow cytometry assay. Reumatologia. 2015;53(2):74-78. doi: 10.5114/reum.2015.51506
3. Brewerton DA, Hart FD, Nicholls A, Caffrey M, James DC, Sturrock RD: Ankylosing spondylitis and HL-A27. Lancet. 1973 Apr 28;1(7809):904-907
4. Albrecht J, Muller HA: HLA-B27 typing by use of flow cytofluorometry. Clin Chem. 1987 Sep;33(9):1619-1623
Anti-HLA fluorescein isothiocyanate (FITC)/CD3 phycoerythrin
Monday through Saturday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
86812
Test Id | Test Order Name |
Order LOINC Value
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
|
---|---|---|
LY27B | HLA-B27, B | 26028-1 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
LY27 | HLA-B27 Result | 26028-1 |
B27C | Interpretation | 96625-9 |