Test Catalog

Test ID: MPNCM    
Myeloproliferative Neoplasm, CALR with Reflex to MPL, Varies

Useful For Suggests clinical disorders or settings where the test may be helpful

Aiding in the distinction between a reactive cytosis and a myeloproliferative neoplasm when JAK2V617F testing result is negative

Reflex Tests Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test IDReporting NameAvailable SeparatelyAlways Performed
MPNMLMPL Exon 10 Sequencing, ReflexNo, (bill only)No

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test reflexively evaluates for variants in the CALR and MPL genes commonly associated with BCR/ABL1-negative myeloproliferative neoplasms. The testing sequence is based on the reported frequency of gene variants in this disease group. It is usually ordered when a JAK2 V617F result is known to be negative. Initial testing evaluates for the presence of the CALR insertions and deletions. If out-of-frame CALR insertions or deletions are detected, the testing algorithm ends. If the CALR result is negative or an in-frame CALR insertion or deletion is identified, then testing proceeds, at an additional charge, to evaluate for variants in exon 10 of the MPL gene by Sanger sequencing. An integrated report is issued with the summary of test results.


The following algorithms are available in Special Instructions:

-Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation

-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation

Special Instructions Library of PDFs including pertinent information and forms related to the test

Method Name A short description of the method used to perform the test

Polymerase Chain Reaction (PCR) and Fragment Analysis

NY State Available Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name Lists a shorter or abbreviated version of the Published Name for a test

MPN (CALR, MPL) Reflex

Aliases Lists additional common names for a test, as an aid in searching

Essential Thrombocythemia
JAK2-negative Myeloproliferative Neoplasm
Janus kinase 2 gene
MPL S505
MPL W515
Myeloproliferative Disorder
Myeloproliferative leukemia virus oncogene
Myeloproliferative Neoplasm (MPN)
Primary Myelofibrosis
Tyrosine Kinase Mutation