Test Catalog

Test ID: MPNR    
Myeloproliferative Neoplasm, JAK2 V617F with Reflex to CALR and MPL, Varies

Useful For Suggests clinical disorders or settings where the test may be helpful

Aiding in the distinction between a reactive cytosis and a chronic myeloproliferative disorder


Evaluating for variants in JAK2, CALR, and MPL genes in an algorithmic process

Reflex Tests Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test IDReporting NameAvailable SeparatelyAlways Performed
CALXCALR, Gene Mutation, Exon 9, ReflexNo, (bill only)No
MPLRMPL Exon 10 Mutation Detection, RNo, (bill only)No

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This reflex test sequentially evaluates for the common major gene variants associated with non-BCR-ABL1-positive myeloproliferative neoplasms until a variant is identified. The testing sequence is based on the reported frequency of gene variants in this disease group. Initial testing evaluates for the presence of the JAK2 V617F variant. If this result is negative or very low positive (0.06%-0.6%), testing proceeds with assessment for CALR  gene variants. If the CALR result is also negative, then testing proceeds to evaluate for variants in exon 10 of the MPL gene. If either JAK2 V617F (>0.6%) or CALR variants are detected in the process, the testing algorithm ends; therefore, the complete reflex is followed only in the event of sequential negative variant. An integrated report is issued with the summary of test results.


The following algorithms are available in Special Instructions:

-Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation

-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation

Special Instructions Library of PDFs including pertinent information and forms related to the test

Method Name A short description of the method used to perform the test

Point Mutation Detection in DNA using Quantitative Polymerase Chain Reaction (PCR)

NY State Available Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name Lists a shorter or abbreviated version of the Published Name for a test

MPN (JAK2 V617F, CALR, MPL) Reflex

Aliases Lists additional common names for a test, as an aid in searching

Janus kinase 2 gene
Tyrosine Kinase Mutation
Essential Thrombocythemia
JAK2-negative Myeloproliferative Neoplasm
Myeloproliferative Disorder
Myeloproliferative Neoplasm (MPN)
Primary Myelofibrosis
MPL S505
Myeloproliferative leukemia virus oncogene