Test Catalog

Test ID: HBEL1    
Hemoglobin Electrophoresis Evaluation, Blood

Useful For Suggests clinical disorders or settings where the test may be helpful

Diagnosis and classification of hemoglobin disorders, including thalassemias and hemoglobin variants

Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test IDReporting NameAvailable SeparatelyAlways Performed
HBELIHb Electrophoresis InterpretationNoYes
HGBCEHb Variant, A2 and F Quantitation,BYesYes
HPLCHPLC Hb Variant, BNoYes

Reflex Tests Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test IDReporting NameAvailable SeparatelyAlways Performed
HPFHHb F Distribution, BNoNo
MASSHb Variant by Mass Spec, BNoNo
SDEXSickle Solubility, BYesNo
IEFIsoelectric Focusing, BNoNo
UNHBHb Stability, BNoNo
ATHALAlpha-Globin Gene AnalysisYesNo
WASQRAlpha Globin Gene Sequencing, BYes, (Order WASEQ)No
WBSQRBeta Globin Gene Sequencing, BYes, (Order WBSEQ)No
WBDDRBeta Globin Cluster Locus Del/Dup,BYes, (Order WBDD)No
WGSQRGamma Globin Full Gene SequencingNoNo
HBEL0Hb Electrophoresis Summary InterpNoNo

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Hemoglobin electrophoresis evaluation will always include hemoglobin A(2) and F and hemoglobin electrophoresis utilizing capillary electrophoresis (CE) and cation exchange high-performance liquid chromatography (HPLC) methods.


Hemoglobin electrophoresis reflex testing, performed at additional charge, may include any or all of the following to identify rare hemoglobin variants present: sickle solubility (hemoglobin S screen); hemoglobin heat and isopropanol stability studies (unstable hemoglobin); isoelectric focusing (IEF), intact globin chain mass spectrometry (hemoglobin variant by mass spectrometry); Hb F distribution by flow cytometry (hemoglobin F distribution); DNA (Sanger) sequencing assays for: 1) beta chain variants and the most common beta thalassemias (beta globin gene sequencing), 2) alpha chain variants and less common non-deletional alpha thalassemias (alpha globin gene sequencing), or 3) gamma chain variants and non-deletional hereditary persistence of fetal hemoglobin (HPFH) (gamma globin full gene sequencing); multiplex ligation-dependent probe amplification (MLPA) assays for: 1) large deletional alpha thalassemias and alpha gene duplications (alpha-globin gene analysis), or 2) beta cluster locus large deletions and duplications, including large deletional HPFH, delta-beta thalassemia (DBT), gamma-delta-beta thalassemia (GDBT), epsilon-gamma-delta-beta thalassemia (EGDBT) and large deletional delta thalassemia. (beta globin cluster locus del/dup),


If test results in the profile are abnormal, results may be reviewed by a hematopathology consultant and a summary interpretation provided.


One or more of the following molecular tests may be reflexed:

ATHAL / Alpha-Globin Gene Analysis, Varies;

WASQR / Alpha-Globin Gene Sequencing, Blood;

WBSQR / Beta-Globin Gene Sequencing, Blood;

WBDDR / Beta-Globin Cluster Locus Deletion/Duplication, Blood;

WGSQR / Gamma-Globin Full Gene Sequencing, Varies;


For cases with molecular testing added, a preliminary interpretation will be reported that discusses the protein test results. After all test results are finalized, an additional consultative interpretation that summarizes all testing and incorporates subsequent genetic results will be provided.


See Benign Hematology Evaluation Comparison in Special Instructions.

Special Instructions Library of PDFs including pertinent information and forms related to the test

Method Name A short description of the method used to perform the test

HBELI: Consultative Interpretation

HGBCE: Capillary Electrophoresis (CE)

HPLC: Cation Exchange/High-Performance Liquid Chromatography (HPLC)

IEF: Isoelectric Focusing (IEF)

MASS: Mass Spectrometry (MS)

HPFH: Flow Cytometry

UNHB: Isopropanol and Heat Stability

HBEL0: Medical Interpretation

NY State Available Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name Lists a shorter or abbreviated version of the Published Name for a test

Hb Electrophoresis Evaluation

Aliases Lists additional common names for a test, as an aid in searching

A2 Hemoglobin
Alpha Globin Variant
Alpha Thalassemia
Barts Hemoglobin
Beta Globin Variant
Beta Thalassemia
H Disease
Hemoglobin A2
Hemoglobin Cascade
Hemoglobin Electrophoresis Cascade Level 1
Hemoglobin Molecular studies
Hemoglobin Variant
HGB (Hemoglobin) Electrophoresis
Isoelectric Focusing
Capillary electrophoresis
High performance liquid chromatography
Mass Spectrometry
Sickle cell
Sickling Test