Test Catalog

Test ID: HAEV1    
Hemolytic Anemia Evaluation, Blood

Useful For Suggests clinical disorders or settings where the test may be helpful

Evaluation of lifelong or inherited hemolytic anemias, including red cell membrane disorders, unstable or abnormal hemoglobin variants, and red cell enzyme disorders


This evaluation is not suitable for acquired causes of hemolysis.

Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test IDReporting NameAvailable SeparatelyAlways Performed
HAEVIHemolytic Anemia InterpretationNoYes
HGBCEHb Variant, A2 and F Quantitation,BYesYes
HPLCHPLC Hb Variant, BNoYes
UNHBHb Stability, BNoYes
FRAGOOsmotic FragilityYes, (Order FRAG)Yes
SCTRLShipping Control VialNoYes
BND3Band 3 Fluorescence Staining, RBCNoYes
G6PDCG6PD Enzyme Activity, BYes, (Order G6PD1)Yes
PKCPK Enzyme Activity, BYes, (Order PK1)Yes
GPICGlucose Phosphate Isomerase, BYes, (Order GPI1)Yes
HKCHexokinase, BYes, (Order HK1)Yes
AKCAdenylate Kinase, BYes, (Order AK1)Yes
PFKCPhosphofructokinase, BYes, (Order PFK1)Yes
PGKCPhosphoglycerate Kinase, BYes, (Order PGK1)Yes
TPICTriosephosphate Isomerase, BYes, (Order TPI1)Yes
GSHGlutathione, BYesYes
P5NTPyrimidine 5' Nucleotidase, BYesYes
PBSMMorphology ReviewNoYes

Reflex Tests Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test IDReporting NameAvailable SeparatelyAlways Performed
SDEXSickle Solubility, BYesNo
IEFIsoelectric Focusing, BNoNo
MASSHb Variant by Mass Spec, BNoNo
HPFHHb F Distribution, BNoNo
ATHALAlpha-Globin Gene AnalysisYesNo
WASQRAlpha Globin Gene Sequencing, BYes, (Order WASEQ)No
WBSQRBeta Globin Gene Sequencing, BYes, (Order WBSEQ)No
WBDDRBeta Globin Cluster Locus Del/Dup,BYes, (Order WBDD)No
WGSQRGamma Globin Full Gene SequencingNoNo
HAEV0Hemolytic Anemia Summary InterpNoNo

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This is a consultative evaluation in which the case will be evaluated and appropriate tests performed, at an additional charge, and the results interpreted. If a peripheral blood smear is provided, the morphologic features will be incorporated into the interpretation.


RBC enzymes will always be performed. Capillary electrophoresis, cation exchange high-performance liquid chromatography (HPLC) and hemoglobin stability studies will always be performed. Reflex testing required to positively identify a hemoglobin abnormality may be added as the case requires. Osmotic fragility (OF) and eosin-5-maleimide (EMA) binding band 3 flow cytometry will be performed on all cases. A normal shipping control for OF is necessary to exclude false-positive results due to preanalytic artifact.


The protein and molecular test results will be released separately which may result in incomplete data until all testing has been finalized.


One or more of the following molecular tests may be reflexed on this test:

-ATHAL / Alpha-Globin Gene Analysis, Varies

-WASQR / Alpha-Globin Gene Sequencing, Blood

-WBSQR / Beta-Globin Gene Sequencing, Blood

-WBDDR / Beta-Globin Cluster Locus Deletion/Duplication, Blood

-WGSQR / Gamma-Globin Full Gene Sequencing, Varies


An additional comprehensive consultative interpretation that summarizes all results will be provided after all tests are completed to incorporate results into an overall evaluation.

Special Instructions Library of PDFs including pertinent information and forms related to the test

Method Name A short description of the method used to perform the test

HAEVI: Medical Interpretation

HGBCE: Capillary Electrophoresis

HPLC: Cation Exchange/High-Performance Liquid Chromatography (HPLC)

UNHB: Isopropanol and Heat Stability

FRAGO: Osmotic Lysis

BND3: Flow Cytometry

G6PDC, PKC, GPIC, HKC, AKC, PFKC, PGKC, TPIC, GSH, P5NT: Kinetic Spectrophotometry (KS)

PBSM: Consultant Review

MASS: Mass Spectrometry (MS)

HPFH: Flow Cytometry

IEF: Isoelectric Focusing

HAEV0: Medical Interpretation

NY State Available Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name Lists a shorter or abbreviated version of the Published Name for a test

Hemolytic Anemia Evaluation

Aliases Lists additional common names for a test, as an aid in searching

EMA binding
Eosin 5 maleimide
Hemolytic Anemia
Hereditary pyropoikilocytosis
Hereditary spherocytosis
Neonatal anemia
Neonatal hemolysis
Pyruvate kinase deficiency
RBC enzyme
RBC membrane
Unstable hemoglobin