Test Id : LPSBF
Lipase, Body Fluid
Useful For
Suggests clinical disorders or settings where the test may be helpful
Determining whether pancreatic inflammation or pancreatic fistula may be contributing to a pathological accumulation of fluid
Method Name
A short description of the method used to perform the test
Enzymatic Colorimetric Reaction
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Specimen Type
Describes the specimen type validated for testing
Body Fluid
Necessary Information
1. Date and time of collection are required.
2. Specimen source is required.
ORDER QUESTIONS AND ANSWERS
| Question ID | Description | Answers |
|---|---|---|
| FLD7 | Fluid Type, Lipase |
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Specimen Type: Body fluid
Preferred Sources:
-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)
-Pleural fluid (pleural, chest, thoracentesis)
-Drain fluid (drainage, Jackson Pratt [JP] drain)
-Pericardial
Acceptable Source: Write in source name with source location (if appropriate)
Collection Container/Tube: Sterile container
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge to remove any cellular material and transfer into a plastic vial.
2. Indicate the specimen source and source location on label.
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the testing laboratory. The minimum volume is sufficient for one attempt at testing.
0.5 mL
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Cerebrospinal fluid, feces, breast milk, saliva, nasal secretions, gastric secretions, bronchoalveolar lavage (BAL) or bronchial washings, sputum, synovial, colostomy/ostomy, urine, or vitreous fluid | Reject |
Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Body Fluid | Refrigerated (preferred) | 7 days | |
| Ambient | 24 hours | ||
| Frozen | 30 days |
Useful For
Suggests clinical disorders or settings where the test may be helpful
Determining whether pancreatic inflammation or pancreatic fistula may be contributing to a pathological accumulation of fluid
Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Lipases are enzymes that hydrolyze glycerol esters of long-chain fatty acids and produce fatty acids and 2-acylglycerol. The pancreas is the primary source of serum lipase. Pancreatic injury results in increased serum lipase levels. Serum lipase is measured to aid in the diagnosis of pancreatitis.
Peritoneal fluid:
The digestive enzymes amylase and lipase can be measured in the identification of pancreatic fluid in the peritoneal cavity. Concentrations are expected to be elevated and at least several-fold times higher in fluid of pancreatic origin compared to simultaneous concentrations in serum.(1,2)
Drain fluid:
Lipase is expected to be elevated in drain fluids formed due to chronic pancreatitis or formation of a fistula following surgery.(1,3,4) Comparison to serum concentrations is recommended with elevations several-fold higher than blood being suggestive of the presence of pancreatic fluid in the drained cavity.(5)
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
An interpretive report will be provided.
Interpretation
Provides information to assist in interpretation of the test results
Fluids (peritoneal, drain):
Lipase concentrations several-fold higher than serum lipase concentrations is suggestive of the presence of pancreatic fluid in the drained cavity.
All other fluids:
Body fluid lipase activity may become elevated due to the presence of pancreatic fluid in the drained cavity. Results should be interpreted in conjunction with serum lipase and other clinical findings.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
In very rare cases of gammopathy, in particular type IgM (Waldenstrom macroglobulinemia) may cause unreliable results.
Calcium dobesilate causes artificially low lipase results.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1. Block DR, Florkowski CM. Body Fluids. In: Rifai N, Horvath AR, Wittwer CT. eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier;2018:chap 43
2. Robert JH, Meyer P, Rohner A. Can serum and peritoneal amylase and lipase determinations help in the early prognosis of acute pancreatitis? Ann Surg. 1986;203(2):163-168. doi:10.1097/00000658-198602000-00009
3. Lipsett PA, Cameron JL. Internal pancreatic fistula. Am J Surg. 1992;163(2):216-220. doi:10.1016/0002-9610(92)90104-y
4. Kaman L, Behera A, Singh R, Katariya RN. Internal pancreatic fistulas with pancreatic ascites and pancreatic pleural effusions: recognition and management. ANZ J Surg. 200;71(4):221-225. doi:10.1046/j.1440-1622.2001.02077.x
5. Sileo AV, Chawla SK, LoPresti PA: Pancreatic ascites: Diagnostic importance of ascitic lipase. Am J Dig Dis. 1975 Dec;20(12):1110-1114. doi:10.1007/BF01070753
6. Nandakumar V, Dolan C, Baumann NA, et al. Effect of pH on the quantification of body fluid analytes for clinical diagnostic testing. Am J Clin Path. 2019; 152(1):S10-S11
Method Description
Describes how the test is performed and provides a method-specific reference
The chromogenic lipase substrate 1,2-O-dilauryl-rac-glycero-3-glutaric acid-(6-methylresorufin) ester is cleaved by the catalytic action of alkaline lipase solution to form 1,2-O-dilauryl-rac-glycerol and an unstable intermediate, glutaric acid-(6-methylresorufin) ester.
This decomposes spontaneously in alkaline solution to form glutaric acid and methylresorufin. Addition of detergent and colipase increases the specificity of the assay for pancreatic lipase.(Package insert: Roche Lipase reagent. Roche Diagnostics; V 3.0, 03/2022)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.
Monday through Sunday
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location
Indicates the location of the laboratory that performs the test
Fees :
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their account representative. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.
CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
CPT codes are provided by the performing laboratory.
CPT codes are provided by the performing laboratory.
83690
LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
| Test Id | Test Order Name | Order LOINC Value |
|---|---|---|
| LPSBF | Lipase, BF | 15212-4 |
| Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
|---|---|---|
| LPBF | Lipase, BF | 15212-4 |
| FLD7 | Fluid Type, Lipase | 14725-6 |