TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: HGH    
Growth Hormone, Serum

Useful For Suggests clinical disorders or settings where the test may be helpful

Diagnosis of acromegaly and assessment of treatment efficacy when interpreted in conjunction with results from glucose suppression test

 

Diagnosis of human growth hormone deficiency when interpreted in conjunction with results from growth hormone stimulation test

 

This test is not intended for use as a screen for acromegaly.

 

This test has limited value in assessing growth hormone secretion in normal children.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

The anterior pituitary secretes human growth hormone (hGH) in response to exercise, deep sleep, hypoglycemia, and protein ingestion. hGH stimulates hepatic insulin-like growth factor-1 and mobilizes fatty acids from fat deposits to the liver. Hyposecretion of hGH causes dwarfism in children. Hypersecretion causes gigantism in children or acromegaly in adults.

 

Because hGH levels in normal and diseased populations overlap, hGH suppression and stimulation tests are needed to evaluate conditions of hGH excess and deficiency; random hGH levels are inadequate.

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Adults

Males: 0.01-0.97 ng/mL

Females: 0.01-3.61 ng/mL

Reference intervals have not been formally verified in-house for pediatric and adolescent patients. The published literature indicates that reference intervals for adult, pediatric, and adolescent patients are comparable.

 

For SI unit Reference Values, see International System of Units (SI) Conversion

Interpretation Provides information to assist in interpretation of the test results

Acromegaly: For suppression testing, normal subjects have a nadir human growth hormone (hGH) concentration  below 0.3 ng/mL after ingestion of a 75-gram glucose dose. Patients with acromegaly fail to show normal suppression. Using the Access ultrasensitive hGH assay, a cutoff of 0.53 ng/mL for nadir hGH was found to most accurately differentiate patients with acromegaly in remission from active disease with a sensitivity of 97% (95% CI, 83%-100%) and a specificity of 100% (95% CI, 82%-100%).(1)

 

Deficiency: A normal response following stimulation tests is a peak hGH concentration above 5 ng/mL in children and above 4 ng/mL in adults. For children, some experts consider hGH values between 5 ng/mL and 8 ng/mL equivocal and only GH peak values greater than8 ng/mL as truly normal. Low levels, particularly under stimulation, indicate hGH deficiency.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

As the hGH test has limited value in assessing growth hormone secretion in normal children, IGF1I / Insulin-Like Growth Factor 1, Serum is recommended as the first test for assessing deficient or excess growth during childhood and adolescent development. IGF1 reference intervals for Tanner stages are available. Suspected causes of dwarfism should be diagnosed with the aid of provocative testing.

 

Elevated levels of human growth hormone indicate the possibility of gigantism or acromegaly, but must be confirmed with stimulation and suppression testing.

 

Growth hormone is secreted in surges; single measurements are of limited diagnostic value.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Bancos I, Algeciras-Schimnich A, Woodmansee WW, et al: Determination of nadir growth hormone concentration cutoff following oral glucose tolerance testing using the Beckman Coulter ultrasensitive growth hormone assay patients with newly diagnosed acromegaly, acromegaly in remission, and healthy subjects. Endocr Pract 2013 Jun 27:1-26

2. Camacho-Hubner C: Assessment of growth hormone status in acromegaly: what biochemical markers to measure and how? Growth Hormone IGF Res 2000;10 Suppl B:S125-299

3. Nilsson AG: Effects of growth hormone replacement therapy on bone markers and bone mineral density in growth hormone-deficient adults. Horm Res 2000;54 Suppl 1:52-57

4. Strasburger CJ, Dattani MT: New growth hormone assays: potential benefits. Acta Paediatr 1997 Nov;Suppl 423:5-11

5. Okada S, Kopchick JJ: Biological effects of growth hormone and its antagonist. Trends Mol Med 2001Mar;7:126-132

6. Veldhuis JD, Iranmanesh A: Physiological regulation of human growth hormone (GH)-insulin-like growth factor type I (IGF-I) axis: predominant impact of age, obesity, gonadal function, and sleep. Sleep 1996;19:S221-224

7. Melmed S: Pathogenesis and diagnosis of growth hormone deficiency in adults. N Engl J Med 2019 Jun 27;380(26):2551-2562. doi: 10.1056/NEJMra1817346