Test Catalog

Test ID: TSH    
Thyroid-Stimulating Hormone (TSH), Beta Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Part of a panel of immunostains used in the classification of pituitary adenomas

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Thyroid-stimulating hormone (TSH) stimulates thyroid growth and production of thyroid hormones. TSH-producing cells constitute approximately 5% of the cells of the normal anterior pituitary. Antibodies to TSH are used in a panel to subclassify pituitary adenomas.

Interpretation Provides information to assist in interpretation of the test results

This test does not include pathologist interpretation; only technical performance of the stain is performed. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut fresh.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Molitch ME: Diagnosis and Treatment of Pituitary Adenomas. A review. JAMA Feb 7;317(5):516-524. doi: 10.1001/jama.2016.19699

2. Mete O, Gomez-Hernandez K, Kucharczyk W, et al: Silent Subtype 3 pituitary adenomas are not always silent and represent poorly differentiated monomorphous plurihormonal Pit-1 lineage adenomas. Modern Pathology 2016 Feb;29(2):131-142. doi: 10.1038/modpathol.2015.151

3. Cossu G, Daniel RT, Pierzchala K, et al: Thyrotropin-secreting pituitary adenomas: a systematic review and meta-analysis of postoperative outcomes and management. Pituitary 2019 Feb;22(1):79-88. doi: 10.1007/s11102-018-0921-3