Test Catalog

Test ID: TRYPN    
Trypsin Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Identifying cells with acinar differentiation in the pancreas

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Trypsinogen is an enzyme involved in protein metabolism that is made by the acinar cells of the exocrine pancreas. After secretion into the small intestine, it is cleaved to its active form, trypsin. In normal pancreas, the antibody stains cells within acini. Ductal cells and islet cells are negative. The antibody to trypsin can be useful in classifying carcinomas of the pancreas by identifying cells with acinar differentiation. Carcinomas with ductal or endocrine differentiation will generally be negative.

Interpretation Provides information to assist in interpretation of the test results

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Bohe H, Bohe M, Lindstrom C, et al: Immunohistochemical demonstration of pancreatic secretory trypsin inhibitor in normal and neoplastic colonic mucosa. J Clin Pathol 1990 Nov;43(11):901-904

2. Bohe H, Bohe M, Jonsson P, et al: Quantification of pancreatic secretory trypsin inhibitor in colonic carcinoma and normal adjacent colonic mucosa. J Clin Pathol 1992 Dec;45(12):1066-1069

3. Marchbank T, Chinery R, Hanby AM, et al: Distribution and expression of pancreatic secretory trypsin inhibitor and its possible role in epithelial restitution. Am J Pathol 1996 Mar;148(3):715-722