Test Catalog

Test ID: THYR    
Thyroglobulin Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Classification of thyroid carcinoma

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Thyroglobulin is a glycoprotein product of thyroid epithelial cells, which is then complexed with iodine before being secreted as thyroid hormone in the blood. In normal thyroid, thyroglobulin staining is seen at the apical surface of thyrocytes and within the colloid in the center of thyroid follicles. The thyroglobulin antibody is useful in classifying poorly differentiated or metastatic thyroid carcinomas.

Interpretation Provides information to assist in interpretation of the test results

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Albores-Saavedra J, Nadji M, Civantos F, Morales AR: Thyroglobulin in carcinoma of the thyroid: an immunohistochemical study. Hum Pathol 1983;14(1):62-66

2. Bejarano PA, Nikiforov YE, Swenson ES, Biddinger PW: Thyroid transcription factor-1, thyroglobulin, cytokeratin 7, and cytokeratin 20 in thyroid neoplasm. Appl Immunohistochem Mol Morphol 2000;8(3):189-194

3. Rosai J: Immunohistochemical markers of thyroid tumors: significance and diagnostic applications. Tumor 2003;89(5):517-519