Test Catalog

Test ID: TCRF1    
T-Cell Receptor Beta (TCR Beta F1) Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Classification of lymphomas

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

T-cell receptor beta (TCR Beta F1) antibody is directed against the beta chain of the alpha/beta T-cell receptor, thus staining the majority of T lymphocytes. Staining is localized at cell membranes with weak cytoplasmic staining in some cells. Positive staining in malignant lymphomas can confirm T-cell lineage and further subtype as alpha/beta T cells.

Interpretation Provides information to assist in interpretation of the test results

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Chan WC, Borowitz MJ, Hammami A, et al: T-cell receptor antibodies in the immunohistochemical studies of normal and malignant lymphoid cells. Cancer 1988;62(10):2118-2124

2. Chuang S, Young-Hyeh K: Cutaneous nonmycotic T- and natural killer/T-cell lymphomas: diagnostic challenges and dilemmas. J Am Acad Dermatol 2014;70:724-735

3. Krajewski AS, Myskow MW, Salter DM, et al: Diagnosis of T-cell lymphoma using beta F1, anti-T-cell receptor beta chain antibody. Histopathology 1989:15(3):239-247

4. Rudiger T, Weisenburger DD, Anderson JR, et al: Peripheral T-cell lymphoma (excluding anaplastic large-cell lymphoma): results from the Non-Hodgkin's Lymphoma Classification Project. Annals of Oncol 2002;13:140-149