Test Catalog

Test ID: NUT    
NUT Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Aiding in the diagnosis of nuclear protein in testis (NUT) midline carcinoma

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Nuclear protein in testis (NUT) is normally confined to the germ cells of the testis and ovary. A recently recognized cancer is NUT midline carcinoma (NMC), defined by the presence of chromosomal rearrangements involving the NUT gene on chromosome 15q14. NMCs are aggressive and highly lethal carcinomas, and are very difficult to discern from other poorly differentiated carcinomas by morphology alone.

Interpretation Provides information to assist in interpretation of the test results

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Davis BN, Karabakhtsian RG, Pettigrew AL, et al: Nuclear protein in testis midline carcinomas. A lethal and under recognized entity. Arch Pathol Lab Med 2011;135:1494-1498

2. Evans AG, French CA, Cameron MJ, et al: Pathologic characteristics of NUT midline carcinoma arising in the mediastinum. Am J Surg Pathol 2012;36(8):1222-1227

3. French CA: NUT midline carcinoma. Cancer Genet Cytogenet 2010;203:16-20

4. Haack H, Johnson LA, Fry CJ, et al: Diagnosis of NUT midline carcinoma using a NUT-specific monoclonal antibody. Am J Surg Pathol 2009;33(7):984-991

5. Stelow EB: A review of NUT midline carcinoma. Head Neck Pathol 2011;5:31-35