Test Catalog

Test ID: MYC    
MYC Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Assessment of MYC expression

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

MYC is a proto-oncogene commonly overexpressed in many malignant neoplasms, including some B-cell lymphomas. MYC translocations are a hallmark abnormality of Burkitt lymphoma. The presence of the MYC translocation may be a helpful indicator of poor prognosis or an aggressive clinical course in diffuse large B-cell lymphoma.

Interpretation Provides information to assist in interpretation of the test results

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut fresh.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Mentzel T, Schildhaus HU, Palmedo G, et al: Postradiation cutaneous angiosarcoma after treatment of breast carcinoma is characterized by MYC amplification in contrast to atypical vascular lesions after radiotherapy and control cases: clinicopathological, immunohistochemical and molecular analysis of 66 cases. Mod Pathol 2012;25(1):75-85

2. Faria M, Khayat A, Burbano R, et al: c-MYC amplification and expression in astrocytic tumors. Acta Neuropathol 2008;116:87-95

3. Ruzinova M, Caron T, Rodig S: Altered subcellular localization of c-Myc protein identifies aggressive B-cell lymphomas harboring a c-MYC translocation. Am J Surg Pathol 2010;34(6):882-891