Test Catalog

Test ID: HPYL    
Helicobacter pylori (H pylori) Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Aiding in the identification of Helicobacter pylori infection

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Helicobacter pylori is a bacterium that frequently infects the stomach, colonizing the gastric pits. H pylori infection is associated with the development of gastroduodenal ulcers and gastric mucosa-associated lymphoid tissue (MALT) lymphomas.

Interpretation Provides information to assist in interpretation of the test results

This test does not include pathologist interpretation; only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request., call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Patnayak R, Reddy V, Jena A, et al: Helicobacter pylori in cholecystectomy specimens-morphological and immunohistochemical assessment. J Clin Diagn Res. 2016 May;10(5):EC01-3. doi: 10.7860/JCDR/2016/14802.7716

2. Son JH, Lebwohl B, Sepulveda AR, Lagana SM: Utilization rate of Helicobacter pylori immunohistochemistry is not associated with the diagnostic rate of helicobacter pylori infection. Appl Immunohistochem Mol Morphol. 2019 Oct;27(9):694-698. doi: 10.1097/PAI.0000000000000680

3. Lee JY, Kim N: Diagnosis of Helicobacter pylori by invasive test: histology. Ann Transl Med. 2015 Jan;3(1):10. doi: 10.3978/j.issn.2305-5839.2014.11.03