Test Catalog

Test ID: HRPV8    
Human Herpes Virus, Type 8 (HHV-8) Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Identification of human herpes virus type 8 infection

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Human herpes virus type 8 (HHV-8) infection can lead to the development of lymphoproliferative diseases or other neoplasms, especially in the setting of HIV. These neoplasms include the plasma cell variant of Castleman disease, Kaposi sarcoma, and primary effusion lymphoma.

Interpretation Provides information to assist in interpretation of the test results

This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Beukes CA: The incidence of human herpes virus-8 expression in lymph node biopsies from human immunodeficiency virus-positive patients. Thiart J.Histopathology. 2012 Nov;61(5):942-944. doi: 10.1111/j.1365-2559.2012.04291.x

2. Roe CJ, Siddiqui MT, Lawson D, Cohen C: RNA In situ hybridization for Epstein-Barr virus and cytomegalovirus: Comparison with In situ hybridization and immunohistochemistry. Appl Immunohistochem Mol Morphol. 2019 Feb;27(2):155-159. doi: 10.1097/PAI.0000000000000568

3. Speicher DJ, Wanzala P, D'Lima M, et al: Diagnostic challenges of oral and cutaneous Kaposi's sarcoma in resource-constrained settings. J Oral Pathol Med. 2015 Nov;44(10):842-9. doi: 10.1111/jop.12315