Test Catalog

Test ID: CYC1    
Cyclin D1 Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Classification of low-grade B-cell lymphomas

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Cyclin D1 is a protein that regulates entry of the cell into cell cycle. It drives the transition between G0 and G1 phase. In normal tissues, basal epithelial cells, endothelial cells, and stromal cells are often cyclin D1 positive. As a result of a translocation involving the cyclin D1 gene and IgH, t(11;14), the vast majority of mantle cell lymphomas overexpress cyclin D1. This is a useful feature in the classification of low-grade B-cell lymphomas.

Interpretation Provides information to assist in interpretation of the test results

This test does not include pathologist interpretation; only technical performance of the stain is performed. If an interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut fresh.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Salem Amir M, et al:  Prognostic value of combined; Cox-2, cyclin D1 and P21 expression in colorectal cancer (CRC) patients: An immunohistochemical study. Open J Pathol. 2018;8(3):106-121

2. Parvin T, et al: Prognostic utility of cyclin D1 in invasive breast carcinoma. Indian J Surg Oncol. 2019;10(1):167-173

3. Li Z, et al: Prognostic significance of cyclin D1 expression in renal cell carcinoma: a systematic review and meta-analysis. Pathol Oncol Res. 2020;26(3):1401-1409