Test Catalog

Test ID: CMVI    
Cytomegalovirus (CMV) Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Identification of cytomegalovirus infection

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Cytomegalovirus (CMV) stain visualizes the intranuclear and cytoplasmic viral inclusions of CMV-infected cells. CMV can cause severe systemic infection (primary or reactivated infection) in patients who are immunocompromised. Antibodies to cytomegalovirus fail to react with any normal human tissue.

Interpretation Provides information to assist in interpretation of the test results

This test does not includes pathologist interpretation; only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Johnson J, Affolter K, Boynton K, et al: CMV disease in IBD: Comparison of diagnostic tests and correlation with disease outcome. Inflamm Bowel Dis. 2018 Jun 8;24(7):1539-1546. doi: 10.1093/ibd/izy045

2. Agnarsdottir M, Popova S, Alafuzoff I: Expression of CMV protein pp65 in cutaneous malignant melanoma. PLoS One. 2019 Oct 11;14(10):e0223854. doi: 10.1371/journal.pone.0223854

3. Jung KH, Kim J, Lee HS, et al: Clinical Implications of the CMV-Specific T-Cell Response and Local or Systemic CMV Viral Replication in Patients With Moderate to Severe Ulcerative Colitis. Open Forum Infect Dis. 2019 Dec 18;6(12):ofz526. doi: 10.1093/ofid/ofz526