Test Catalog

Test ID: CMVI    
Cytomegalovirus (CMV) Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Aids in the identification of cytomegalovirus infection

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Cytomegalovirus (CMV) stain visualizes the intranuclear and cytoplasmic viral inclusions of CMV-infected cells. CMV can cause severe systemic infection (primary or reactivated infection) in immunocompromised hosts. Antibodies to cytomegalovirus fail to react with any normal human tissue.

Interpretation Provides information to assist in interpretation of the test results

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut fresh.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Colina F, Juca NT, Moreno E, et al: Histological diagnosis of cytomegalovirus hepatitis in liver allografts. J Clin Pathol 1995;48:351-357

2. Niedobitek G, Finn T, Herbst H, et al: Detection of cytomegalovirus by In situ hybridisation and immunohistochemistry using new monoclonal antibody CCH2: A Comparison of Methods. J Clin Pathol 1988;41(9):1005-1009

3. Strickler JG, Manivel JC, Copenhaver CM, Kubic VL: Comparion of In situ hybridization and immunohistochemistry for detection of cytomegalovirus and herpes simplex virus. Human Pathology 1990;21(4):443-448