Test Catalog

Test ID: CD57    
CD57 Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Marker of natural killer cells and a subset of follicular T helper cells


Aids in the identification of tumors of neuroectodermal origin

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

CD57 is a 110-kD glycoprotein that is selectively expressed on natural killer cells and a subset of follicular T helper cells. Antibodies to CD57 also stain the myelin sheaths of central and peripheral nervous system cells. In normal tonsil, CD57 will stain a minor population of lymphocytes in the germinal centers. It is immunoreactive in tumors of neuroectodermal origins such as small cell carcinoma of the lung and carcinoid tumors, and is positive in adenocarcinomas of the prostate as well as normal and hyperplastic prostatic epithelium. Increased numbers of CD57-positive T cells are present in the background of lymphocyte-predominant Hodgkin lymphoma.

Interpretation Provides information to assist in interpretation of the test results

This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut fresh.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Boudova L, Torlakovic E, Delabie J, et al: Nodular lymphocyte-predominant Hodgkin lymphoma with nodules resembling T-cell/histiocyte-rich B-cell lymphoma: differential diagnosis between nodular lymphocyte-predominant Hodgkin lymphoma and T-cell/histiocyte-rich B-cell lymphoma. Blood 2003;102(10):3753-3758

2. Khan A, Baker SP, Patwardhan NA, Pullman JM: CD57 (Leu-7) expression is helpful in diagnosis of the follicular variant of papillary thyroid carcinoma. Virchows Arch 1998;432(5):427-432

3. Khoury T, Chandrasekhar R, Wilding G, et al: Tumour eosinophilia combined with an immunohistochemistry panel is useful in the differentiation of type B3 thymoma from thymic carcinoma. Int J Exp Pathol 2011;92(2):87-96

4. Wick MR: Immunohistology of neuroendocrine and neuroectodermal tumors. Semin Diagn Pathol 2000;17(3):194-203