Test Catalog

Test ID: CD15    
CD15 Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Phenotyping leukemias and lymphomas

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

CD15 is expressed in granulocytes and can be expressed in malignant lymphomas and acute myeloid leukemias. The Reed-Sternberg cells of classical Hodgkin lymphoma are characteristically positive for CD15 and CD30.

Interpretation Provides information to assist in interpretation of the test results

This test does not include pathologist interpretation; only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Benharroch D, Pilosof S, Gopas J, Levi I: Primary refractory and relapsed classical Hodgkin lymphoma - Significance of differential CD15 expression in Hodgkin-Reed-Sternberg cells. J Cancer. 2012;3: 322-327

2. Roge R, Nielsen S, Vyberg M: Carb-3 is the superior antiCD15 monoclonal antibody for immunohistochemistry. Appl Immunohistochem Mol Morphol. 2014; 22(6): 449-458

3. Venkataraman G, Raffeld M, Pittaluga S, et al: CD15-expressing nodular lymphocyte-predominant Hodgkin lymphoma. Histopathology. 2011;5:803-805