Test Catalog

Test ID: BRAFV    
BRAF V600E Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Identification of BRAF V600E-mutated protein

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

BRAF is a serine/threonine protein kinase and a member of the Raf family. The BRAF V600E alteration leads to constitutive activation of the mitogen activated protein kinase pathway, which plays a role in cell proliferation and tumorigenesis. This genetic alteration has been detected in a variety of tumors such as melanoma, colorectal cancer, papillary thyroid carcinoma, hairy cell leukemia, Langerhans cell histiocytosis, and pleomorphic xanthoastrocytomas.

Interpretation Provides information to assist in interpretation of the test results

This test does not include pathologist interpretation: only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Ida C, Vrana JA, Rodriguez FJ, et al: Immunohistochemistry is highly sensitive and specific for detection of BRAF V600E mutation in pleomorphic xanthoastrocytoma. Acta Neuopath communications 2013;1:20

2. Capper D, Berghoff AS, Magerle M, et al: Immunohistochemical testing of BRAF V600E status in 1,120 tumor tissue samples of patients with brain metastases. Acta Neuropathol 2012;123(2):223-233

3. Andrulis M, Penzel R, Weichert W, et al: Application of a BRAF V600E Mutation-specific Antibody for the Diagnosis of Hairy Cell Leukemia. Am J Surg Pathol 2012;36(12):1796-1800

4. Koperek O, Kornauth C, Capper D, et al: Immunohistochemical detection of the BRAF V600E-mutated protein in papillary thyroid carcinoma. Am J Surg Pathol 2012;36(6):844-850

5. Skorokhod A, Capper D, von Deimling A, et al: Detection of BRAF V600E mutations in skin metastases of malignant melanoma by monoclonal antibody VE1. J Am Acad skin metastases of malignant melanoma by monoclonal antibody VE1. J Am Acad Dermatol 2012;67(3):488-491