Test Catalog

Test ID: SATB2    
SATB2 Immunostain, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Identification of colorectal carcinoma and rectal neuroendocrine tumors

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Special AT-rich sequence binding protein 2 (SATB2) is a transcription factor that regulates gene expression through chromatin remodeling. SATB2, when used in combination with the marker Keratin 20, may identify more than 95% of colorectal carcinoma. It may also be used to differentiate rectal neuroendocrine tumors from other neuroendocrine tumors of the gastrointestinal tract.

Interpretation Provides information to assist in interpretation of the test results

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.


The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Berg KB, Schaeffer DF: SATB2 as an Immunohistochemical Marker for Colorectal Adenocarcinoma, A concise Review of Benefits and Pitfalls. Arch Pathol Lab Med 2017;141(10):1428-1433

2. Davis JL, Horvai AE: Special AT-rich sequence-binding protein 2 (SATB2) expression is sensitive but may not be specific for osteosarcoma as compared with other high-grade primary bone sarcomas. Histopathology 2016;69(1):84-90

3. Dragomir A, de Wit M, Johansson C, et al: The role of SATB2 as a diagnostic marker for tumors of colorectal origin. Am J Clin Pathol 2014;141(5):630-638

4. Magnusson K, de Wit M, Brennan DJ, et al: SATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinoma. Am J Surg Pathol 2011;35(7):937-948