Detecting the presence and titer of a specific factor inhibitor directed against coagulation factor VIII
This test is not useful for detecting the presence of inhibitors directed against other clotting factors and will not detect the presence of lupus anticoagulants.
Factor VIII inhibitors are IgG antibodies directed against coagulation FVIII that typically result in development of potentially life-threatening hemorrhage. These antibodies may be alloimmune: developing in patients with congenital FVIII deficiency (hemophilia A) in response to therapeutic infusions of factor VIII concentrate or autoimmune: occurring in nonhemophiliac patients (not previously factor VIII deficient) either spontaneously, during pregnancy, or in association with autoimmune diseases.
The interpretive report will include assay information, background information, and conclusions based on the test results.
1. Verbruggen B, van Heerde WL, Laros-van Gorkom BA: Improvements in Factor VIII Inhibitor Detection: From Bethesda to Nijmegen. Semin Thromb Hemost 2009 Nov;35(8):752-759
2. Miller C, Platt S, Rice A, et al: Validation of Nijmegen-Bethesda assay modifications to allow inhibitor measurement during replacement therapy and facilitate inhibitor surveillance. J Thromb Haemost 2012;10:1055-1061