Test Catalog

Test ID: ECADB    
E-Cadherin Immunostain, Bone Marrow, Technical Component Only

Useful For Suggests clinical disorders or settings where the test may be helpful

Differentiation between lobular and ductal neoplasms of the breast

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Membrane protein expressed on normal breast epithelial cells. Expression can be lost on lobular neoplasms of the breast, in contrast to ductal neoplasms of the breast.

Interpretation Provides information to assist in interpretation of the test results

This test does not include pathologist interpretation: only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

This test is optimized for use on B5-fixed, decalcified, paraffin-embedded bone marrow specimens.


Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Engstrom MJ, Opdahl S, Vatten LJ, et. al: Invasive lobular breast cancer: The prognostic impact of histopathological grade, E-cadherin and molecular subtypes. Histopathol. 2015;66(3):409-419

2. Dabbs DJ, Schnitt SJ, Geyer FC, et al: Lobular neoplasia of the breast revisited with emphasis on the role of e-cadherin immunohistochemistry. Am J Surg Pathol. 2013;37;e1-e11

3. Liu J, Feng C, Deng M, et al: E-cadherin expression phenotypes associated with molecular subtypes in invasive non-lobular breast cancer: evidence from a retrospective study and meta-analysis. World J Surg Onc. 2017;15:139