Test Catalog

Test ID: SCTF    
Sex Chromosome Determination, FISH, Tissue

Useful For Suggests clinical disorders or settings where the test may be helpful

Identifying the sex chromosome complement in paraffin-embedded tissues

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test includes a charge for application of the first probe set (2 fluorescence in situ hybridization probes) and professional interpretation of results.


Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Genotypically normal females possess 2 X chromosomes (XX); genotypically normal males possess 1 X chromosome and 1 Y chromosome (XY). Determining the sex chromosome complement in a tissue specimen can be used to:

-Identify opposite sex-donor cells post-transplant

-Help resolve cases of suspected sample mix-up

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

An interpretive report will be provided.

Interpretation Provides information to assist in interpretation of the test results

An interpretive report will be provided.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

This test is not approved by the U.S. Food and Drug Administration and it is best used as an adjunct to existing clinical and pathologic information.


Fixatives other than formalin (eg, Prefer, Bouin's) may not be successful for FISH assays, however non-formalin-fixed samples will not be rejected.


Paraffin-embedded tissues that have been decalcified are generally unsuccessful for FISH analysis. The pathologist reviewing the hematoxylin and eosin-stained slide may find it necessary to cancel testing.

Supportive Data

Fluorescence in situ hybridization analysis was performed on a series of formalin-fixed, paraffin-embedded specimens. Specimens with known sex chromosome aneusomy were correctly identified.

Clinical Reference Recommendations for in-depth reading of a clinical nature

1. Nakhleh RE, Zarbo RJ: Surgical pathology specimen identification and accessioning: A College of American Pathologists Q-Probes study of 1,004,115 cases from 417 institutions. Arch Pathol Lab Med. 1996 Mar;120:227-233

2. Riopel MA, Yu IT, Hruban RH, Lazenby AJ, Griffin CA, Perlman EJ: Whose tumor is this: FISHing for the answer. Mod Pathol. 1995;8:456-457

3. Valenstein PN, Raab SS, Walsh MK: Identification errors involving clinical laboratories: a College of American Pathologists Q-Probes study of patient and specimen identification errors at 120 institutions. Arch Pathol Lab Med. 2006 Aug;130(8):1106-1113

Special Instructions Library of PDFs including pertinent information and forms related to the test