Test Catalog

Test ID: CULAF    
Culture for Genetic Testing, Amniotic Fluid

Useful For Suggests clinical disorders or settings where the test may be helpful

Producing amniocyte cultures that can be used for genetic analysis

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing amniocytes for prenatal genetic testing.


Once confluent flasks are established, the amniocyte cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Fetal cells obtained by amniocentesis (amniocytes) are used for a wide range of laboratory tests. Prior to testing, the cells may need to be cultured to obtain adequate numbers of amniocytes.

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Not applicable

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Interfering factors:

-Inadequate amount of specimen may not permit adequate analysis

-Exposure of the specimen to temperature extremes (freezing or > 30 degrees C) may destroy cells and interfere with attempts to culture cells

-Improper packaging may result in broken, leaky, and contaminated specimens during transport

-Transport time should not exceed 2 days

-Contamination by maternal cells may interfere with attempts to culture cells and may cause interpretive problems

Clinical Reference Recommendations for in-depth reading of a clinical nature

The AGT Cytogenetics Laboratory Manual Fourth edition. Edited by MS Arsham, MJ Barch, HJ Lawce. John Wiley and Sons Inc, Hoboken, NJ, 2017