Test Catalog

Test Id : SAM

Entamoeba histolytica Antibody, Serum

Useful For
Suggests clinical disorders or settings where the test may be helpful

As an adjunct in the diagnosis of extraintestinal amebiasis, especially liver abscess

Method Name
A short description of the method used to perform the test

Enzyme-Linked Immunosorbent Assay (ELISA)

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

E. histolytica Ab, S

Aliases
Lists additional common names for a test, as an aid in searching

Amebic Antibody

Amoeba Histolytica

Amoeba, Serum

Amoebiasis Antibody

Amebiasis

Amebic Serology

E Histolytica Antibody, Serum

Entamoeba Histolytica Antibodies

Entamoeba Histolytica Serology

Parasite Serologies-done by clinic

SAM

Specimen Type
Describes the specimen type validated for testing

Serum

Ordering Guidance

Direct detection of Entamoeba histolytica in fecal specimens is recommended to diagnose intestinal amebiasis. See OAP / Ova and Parasite, Concentrate and Permanent Smear, Microscopy, Feces or OAPNS / Ova and Parasite, Microscopy, Varies.

Shipping Instructions

 

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

0.15 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject
Gross lipemia Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 30 days
Refrigerated 7 days

Useful For
Suggests clinical disorders or settings where the test may be helpful

As an adjunct in the diagnosis of extraintestinal amebiasis, especially liver abscess

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Amebiasis is an infection by the protozoan parasite, Entamoeba histolytica. The infection is acquired by ingestion of cysts in fecally contaminated food or water; excystation and infection occur in the large intestine. After excystation, trophozoites attach to the intestinal wall and liberate extracellular enzymes that enable invasion of the mucosa and spread to other organs, especially the liver and lung where abscesses develop.

 

Amebiasis (or amebic dysentery) can cause bloody diarrhea accompanied by fever and prostration. White and red blood cells are found in the stool. Liver abscess can develop several weeks to months later producing hepatomegaly and fever.

 

Pathogenic (E histolytica) and nonpathogenic (Entamoeba dispar) species of Entamoeba occur. Additionally, some of those infected with pathogenic strains are asymptomatic cyst carriers.

 

Serology may be particularly useful in supporting the diagnosis of amebic liver abscess in patients without a definite history of intestinal amebiasis and who have not spent substantial periods of time in endemic areas.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Negative

Reference values apply to all ages.

Interpretation
Provides information to assist in interpretation of the test results

A positive result suggests current or previous infection with Entamoeba histolytica.

 

Since pathogenic and nonpathogenic species of Entamoeba cannot be differentiated microscopically, some authorities believe a positive serology indicates the presence of the pathogenic species (ie, E histolytica).

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Previous episodes of intestinal amebiasis may produce a positive serology.

 

Serologic results should be used as an aid in diagnosis and should not be interpreted as diagnostic by themselves.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Bruckner DA: Amebiasis. Clin Microbiol Rev. 1992;5:356-369. doi: 10.1128/CMR.5.4.356

2. Petri WA, Haque R, Moonah SN: Entamoeba species, including amebic colitis and liver abscess. In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020:3273-3286

Method Description
Describes how the test is performed and provides a method-specific reference

Purified antigens are coated to a microwell plate. Antibodies in the patient samples bind to the antigens and are determined during the second step by using enzyme-labelled protein A (the conjugate). The enzyme converts the colorless substrate (urea peroxide/TMB) to a blue end product. The enzyme reaction is stopped by adding sulfuric acid and the colour of the mixture switches from blue to yellow at the same time. The final measurement is carried out at 450 nm on a photometer using a reference wavelength greater than or equal to 620 nm.(Package insert: RIDASCREEN Entamoeba histolytica IgG. R-Biopharm AG; 06/10/2016)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Tuesday, Thursday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 5 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

86753

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports