Test Catalog

Test Id : FIBR

Fibroblast Culture for Biochemical and Molecular Testing, Tissue

Useful For
Suggests clinical disorders or settings where the test may be helpful

Obtaining cultured cells for specialized testing including enzymatic and molecular genetic

Additional Tests
Lists tests that are always performed, at an additional charge, with the initial tests.

Test Id Reporting Name Available Separately Always Performed
CRYOB Cryopreserve for Biochem Studies No Yes

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing skin fibroblasts or other biopsies for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.

 

When this test is ordered, cryopreservation will always be performed at an additional charge. However, for multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered.

 

Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested. To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.

 

If viable cells are not obtained within 30 days, client will be notified.

 

Mycoplasma screening will be performed on all successfully grown fibroblast cultures. If Mycoplasma is detected, all pending fibroblast clinical testing will be cancelled, and the samples will be discarded.

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Name
A short description of the method used to perform the test

FIBR: Cultivated from Biopsy as Monolayer

CRYOB: Fibroblast Subculture followed by Cryopreservation and Storage

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Fibroblast Culture

Aliases
Lists additional common names for a test, as an aid in searching

Fibroblast Culture

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing skin fibroblasts or other biopsies for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.

 

When this test is ordered, cryopreservation will always be performed at an additional charge. However, for multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered.

 

Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested. To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.

 

If viable cells are not obtained within 30 days, client will be notified.

 

Mycoplasma screening will be performed on all successfully grown fibroblast cultures. If Mycoplasma is detected, all pending fibroblast clinical testing will be cancelled, and the samples will be discarded.

Specimen Type
Describes the specimen type validated for testing

Tissue

Ordering Guidance

This test is most useful for skin biopsy specimens. The specimen is cryopreserved for a minimum of 3 years.

 

This test is not appropriate for products of conception (POC) or chorionic villus sampling (CVS) specimens. If this test is ordered for either a POC or CVS specimen, the test will be canceled and CULFB / Fibroblast Culture for Molecular Testing, Chorionic Villi/Products of Conception will be performed as the appropriate test.

 

If ordering this test to obtain DNA for testing at a different laboratory location, see Necessary Information.

Necessary Information

1. Include patient clinical history with request. This information will be used for any testing performed on the cultured cells.

2. Indicate the tests to be performed on the fibroblast culture cells.

Note: For courtesy DNA extraction from fibroblasts for testing performed at locations other than Mayo Clinic Laboratories (MCL), a MCL Test ID and test requisition for the requested laboratory are required. Prior to specimen collection, call Mayo Lab Inquiry at 800-533-1710 to confirm availability of send-out testing options as courtesy DNA extraction will not be performed for tests not in the MCL Referral catalog.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Submit only 1 of the following specimens:

 

Specimen Type: Cultured fibroblasts

Container/Tube: T-75 or T-25 flask

Specimen Volume: 1 Full T-75 flask or 2 full T-25 flasks

Specimen Stability Information: Ambient/Refrigerated

 

Specimen Type: Skin biopsy

Supplies: Fibroblast Biopsy Transport Media (T115)

Container/Tube: Sterile container with any standard cell culture media (eg, minimal essential media, RPMI 1640). The solution should be supplemented with 1% penicillin and streptomycin. Tubes of culture media can be supplied upon request (Eagle's minimum essential medium with 1% penicillin and streptomycin).

Specimen Volume: 4-mm punch

Specimen Stability Information: Refrigerated/Ambient

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Forms

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Tissue in formalin or fixative preservative Reject

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Tissue Varies (preferred)

Useful For
Suggests clinical disorders or settings where the test may be helpful

Obtaining cultured cells for specialized testing including enzymatic and molecular genetic

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing skin fibroblasts or other biopsies for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.

 

When this test is ordered, cryopreservation will always be performed at an additional charge. However, for multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered.

 

Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested. To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.

 

If viable cells are not obtained within 30 days, client will be notified.

 

Mycoplasma screening will be performed on all successfully grown fibroblast cultures. If Mycoplasma is detected, all pending fibroblast clinical testing will be cancelled, and the samples will be discarded.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Cultures of skin fibroblasts are useful for specialized tests requiring skin cells. These cells can be cultured and tested at Mayo Clinic Laboratories or sent to external laboratories performing these specialized tests. In addition, cells are frozen for a minimum of 3 years for potential future studies on cultured cells or for molecular genetic testing.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Not applicable

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Potential interfering factors:

-Lack of viable cells

-Bacterial contamination

-Failure to transport tissue in an appropriate media

-Excessive transport time

-Exposure of the specimen to temperature extremes (freezing or >30 degrees C)

-Improper packaging may result in broken or leaky specimen containers or contamination of specimens during transport

Special Instructions
Library of PDFs including pertinent information and forms related to the test

Method Description
Describes how the test is performed and provides a method-specific reference

Submitted biopsy specimens are minced, placed in a culture flask with additional culture media supplemented with fetal calf serum, and incubated. When the fibroblasts have grown from the pieces of tissue (2-4 weeks), the cultures are expanded and then cryopreserved (CRYOB / Cryopreservation for Biochemical Studies). If specific molecular or biochemical testing is concurrently ordered, cells will be harvested or prepared for indicated testing. Submitted cultured cells are examined for cell integrity and fed fresh media. Once grown to confluency, the cultures are expanded and then, if indicated, cryopreserved.(Unpublished Mayo method)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Varies

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

40 to 45 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

3 years minimum-Check with lab for availability

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

Not Applicable

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

88233-Fibroblast culture

88240-Cryopreservation for biochemical studies

LOINC® Information

Test Id Test Order Name Order LOINC Value
FIBR Fibroblast Culture 96300-9
Result Id Test Result Name Result LOINC Value
Result LOINC Value Tooltip
8482 Fibroblast Culture 96300-9

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports