Test Catalog

Test Id : BRUC

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Brucella Culture, Varies

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Overview

Test Catalog

Useful For
Suggests clinical disorders or settings where the test may be helpful

Diagnosis of brucellosis

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
TISSR Tissue Processing No, (Bill Only) No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged.

Method Name
A short description of the method used to perform the test

Conventional Culture Technique

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Brucella Culture

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged.

Specimen Type
Describes the specimen type validated for testing

Varies

Ordering Guidance

Mayo Clinic Laboratories does not perform species identification of Brucella; these isolates should be submitted to the State Health Department laboratory.

Necessary Information

Specimen source is required.

ORDER QUESTIONS AND ANSWERS

Question ID Description Answers
Q00M0009 Specimen Source

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Bone marrow

Container/Tube: Sterile container

Specimen Volume: 1 mL

Specimen Stability Information: Ambient <24 hours

 

Alternate:

Specimen Type: Abscess, respiratory specimen, spinal fluid, sterile body fluid, or tissue

Container/Tube: Sterile container

Specimen Volume: 1 mL

Specimen Stability Information: Refrigerated <24 hours

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Other Frozen specimen Specimen >24 hours

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Varies Varies

Useful For
Suggests clinical disorders or settings where the test may be helpful

Diagnosis of brucellosis

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Brucella species are facultative intracellular Gram-negative staining bacilli capable of producing the disease "brucellosis" in humans. Human disease likely is acquired by contact with animals infected with the organism (Brucella abortus, B suis, B melitensis, and occasionally B canis) either by direct contact or by ingestion of meat or milk. The signs and symptoms associated with brucellosis may include fever, night sweats, chills, weakness, malaise, headache, and anorexia. The physical examination may reveal lymphadenopathy and hepatosplenomegaly. A definitive diagnosis of brucellosis is made by recovering the organism from blood, fluid (including urine), or tissue specimens.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

No growth after 14 days

Interpretation
Provides information to assist in interpretation of the test results

Isolation of a Brucella species indicates infection.

 

Cultures of blood and/or bone marrow are positive in 70% to 90% of acute Brucella infections, but much less so in subacute or chronic infections. In these latter instances, culture yield is highest from the specific tissue involved, or serology may be necessary to establish diagnosis.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Although Brucella species are relatively resistant to adverse environmental conditions, excessive delay in the transport of material for culture to the laboratory may result in a lower yield.

 

If a significant delay is expected, specimens should be refrigerated.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

1. Cem Gul H, Erdem H: Chapter 228: Brucellosis (Brucella Species). In Douglas and Bennett's Principles and Practice of Infectious Diseases. Eighth edition. Edited by JE Bennett, R Dolin, MJ Blaser. Philadelphia, PA, Elsevier Saunders, 2015. pp 2584-2589

2. Procop GW, Church DL, Hall GS, et al: Chapter 9: Miscellaneous Fastidious Gram-Negative Bacilli. In Koneman's Color Atlas and Textbook of Diagnostic Microbiology. Seventh edition. Philadelphia, Wolters Kluwer, 2017, pp 472-595

Method Description
Describes how the test is performed and provides a method-specific reference

Specimens are inoculated onto buffered charcoal yeast extract agar (BCYE) or GC-Lect agar for urines and respiratory specimens and chocolate blood agar incubated at 35 degrees C with increased carbon dioxide (CO2). Cultures are incubated for 14 days before issuing a negative report. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and/or nucleic acid sequencing of the 16S ribosomal RNA (rRNA) gene are used for identification.(Araj GF: Chapter 49: Brucella. In Manual of Clinical Microbiology. 12th edition. Edited by KC Carroll, MA Pfaller. Washington DC, American Society for Microbiology, 2019, pp 883-892)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

14 to 17 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 day

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their account representative. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

87081-Brucella culture

87077-Identification by MALDI-TOF mass spec (if appropriate)

87153-Aerobe identification by sequencing (if appropriate)

87176-Tissue processing (if appropriate)

LOINC® Information
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.

Test Id Test Order Name Order LOINC Value
BRUC Brucella Culture 552-0
Result Id Test Result Name Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
BRUC Brucella Culture 552-0

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports