Polymerase Chain Reaction (PCR); Sequencing
Daclatasvir resistance
HCV
HCV NS5a Subtype
Ledipasvir resistance
Ombitasvir resistance
Varies
Please submit one of the following:
Plasma:
Specimen Type: Plasma (Preferred)
Container/Tube: EDTA (lavender-top) tube(s).
Specimen volume: 2 mL
Collection Instructions: Draw blood in an EDTA (lavender-top) tube(s). (Plasma gel tube is acceptable.) Spin down and send 2 mL plasma refrigerated in a plastic vial.
Serum:
Specimen Type: Serum
Container/Tube: Red-top tube, serum gel is acceptable.
Specimen volume: 2 mL
Collection Instructions: Draw blood in a plain red-top tube(s). (Serum gel tube is acceptable.) Spin down and send 2 mL serum refrigerated in a plastic vial.
0.6 mL
Hemolysis | Mild OK, Gross reject |
Lipemia | Mild OK, Gross reject |
Icterus | NA |
Other | Heparin plasma |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Refrigerated (preferred) | 14 days | |
Frozen | 42 days | ||
Ambient | 72 hours |
The clinical significance of NS5a resistance associated variants for antiviral therapy may vary according to the clinical status and antiviral treatment experience of the HCV-infected patient.
Testing for NS5a resistance-associated variants prior to initiation of treatment with elbasvir plus grazoprevir in HCV genotype 1a infected patients is recommended.
HCV NS5a Subtype: Not Detected
This assay is designed to amplify HCV genotypes 1a and 1b and may not successfully amplify other HCV genotypes.
This test utilizes RT-PCR and DNA sequencing to detect the presence of treatment-emergent HCV NS5a variants associated with NS5a inhibitor antiviral therapy.
Daily
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
87902
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
FH1N5 | HCV RNA Genotype 1 NS5a Drug Resist | 82525-7 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
Z4846 | HCV NS5a Subtype | 82380-7 |
Z4847 | Daclatasvir Resistance | 82379-9 |
Z4848 | Ledipasvir Resistance | 82377-3 |
Z4849 | Ombitasvir Resistance | 82378-1 |
Z4850 | Elbasvir Resistance | 82376-5 |
Z4851 | Velpatasvir Resistance | 82520-8 |