Detection of human papillomavirus DNA from high-risk genotypes (16, 18, 31, 33, and 51)
Human papillomavirus (HPV) High Risk E6/E7 RNA ISH could be performed and reported at the discretion of the Mayo pathologist if clinically indicated but will not be billed separately.
In Situ Hybridization
HPV (Human Papillomavirus)
HPVHR
GPHPVHRISH
ISHPVFAM16ISH
ISNEGHPVISH
ISDNAHPVISH
Human papillomavirus (HPV) High Risk E6/E7 RNA ISH could be performed and reported at the discretion of the Mayo pathologist if clinically indicated but will not be billed separately.
Special
If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.
The probe set used in this human papillomavirus (HPV) DNA in situ hybridization (ISH) test cannot detect all potential HPV serotypes that are associated with oropharyngeal squamous cell carcinoma. If this test is negative, a more sensitive test, Human Papillomavirus (HPV) High Risk E6/E7 RNA In Situ Hybridization, could be performed and reported if clinically indicated but will not be billed separately.
Attach the green pathology address label included in the kit to the outside of the transport container.
A pathology/diagnostic report and a brief history are required.
Supplies: Pathology Packaging Kit (T554)
Specimen Type: Formalin-fixed, paraffin-embedded tissue block
Specimen Volume: Entire block
Specimen Type: Slides
Slides: 5 unstained glass, positively charged slides with 5 (+ or - 1)-microns formalin-fixed, paraffin-embedded tissue
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763)
-Oncology Test Request (T729)
Wet/frozen tissue Cytology smears Nonformalin fixed tissue Nonparaffin embedded tissue Noncharged slides ProbeOn slides | Reject |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Special | Ambient (preferred) | ||
Refrigerated |
Detection of human papillomavirus DNA from high-risk genotypes (16, 18, 31, 33, and 51)
Human papillomavirus (HPV) High Risk E6/E7 RNA ISH could be performed and reported at the discretion of the Mayo pathologist if clinically indicated but will not be billed separately.
Persistent infections with high-risk human papillomavirus (HPV) genotypes (16, 18, 31, 33, and 51) are associated with cervical, vaginal, vulvar, and head and neck malignancies. Patients with HPV-related oropharyngeal squamous cell carcinoma (OPSCC) have shown better disease-specific survival and overall survival when compared to HPV-negative cases of OPSCC.
This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.
Age of a cut paraffin section can affect staining quality. Stability thresholds vary widely among published literature. Best practice is for paraffin sections to be cut within 6 weeks.
1. Belobrov S, Cornall A, Young R, et al: The role of human papillomavirus in p16-positive oral cancers. J Oral Pathol Med. 2018;47(1):18-24
2. Ducatman B: The role of human papillomavirus in oropharyngeal squamous cell carcinoma. Arch Pathol Lab Med. 2018;142(6):715-718
3. Windon M, D'Souza G, Rettig E, et al: Increasing prevalence of human papillomavirus–positive oropharyngeal cancers among older adults. Cancer. 2018;124(14):2993-2999
In situ hybridization on sections of paraffin-embedded tissue.(Unpublished Mayo method)
Monday through Friday
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.
88365-Primary
88364-If additional ISH
Test Id | Test Order Name | Order LOINC Value |
---|---|---|
HPVHR | HPV High-Risk ONLY DNA ISH | 49896-4 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
71194 | Interpretation | 50595-8 |
71195 | Participated in the Interpretation | No LOINC Needed |
71196 | Report electronically signed by | 19139-5 |
71198 | Material Received | 81178-6 |
71593 | Disclaimer | 62364-5 |
72111 | Case Number | 80398-1 |