Viral identification and confirmation
Test Id | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
VID2 | Additional Testing Virus Ident | No, (Bill Only) | No |
SVIR | Viral Smear, Shell Vial | No, (Bill Only) | No |
When this test is ordered, the reflex tests may be performed and charged.
Shell Vial Assay for Appropriate Virus and/or Tube Cell Culture
50017-VRID2
When this test is ordered, the reflex tests may be performed and charged.
Varies
Suspected isolates of the following viruses will not be accepted at Mayo Clinic Laboratories and should be submitted directly to your state health department or the CDC for confirmatory testing:
-Measles
-Mumps
-Ebola and other causative agents of viral hemorrhagic fevers
-Avian influenza
-Severe Acute Respiratory Syndrome (SARS)
-Middle Eastern Respiratory Syndrome Coronavirus (MERS-CoV)
1. See Infectious Specimen Shipping Guidelines in Special Instructions.
2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.
1. Specimen source is required and must include anatomical site of original specimen collected.
2. Isolate description is required. Include morphology and list any previous tests performed.
Supplies: Infectious Container, Large (T146)
Specimen Type: Pure culture of organism from source cultured
Container/Tube: Cell (viral) culture tube
Specimen Volume: Entire specimen
Collection Instructions:
1. Organism must be in pure culture, actively growing.
2. Completely fill culture tube with appropriate culture medium before shipment.
Hemolysis | NA |
Lipemia | NA |
Icterus | NA |
Other | Agar plate |
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Ambient (preferred) | ||
Refrigerated |
Viral identification and confirmation
When this test is ordered, the reflex tests may be performed and charged.
Viruses are responsible for a broad spectrum of clinical symptoms and diseases. The most commonly isolated viruses are adenovirus, cytomegalovirus (CMV), enteroviruses, herpes simplex virus (HSV), influenza virus, parainfluenza virus (types 1-3), respiratory syncytial virus (RSV), and varicella-zoster virus (VZV).
Some viral infections can be treated with antiviral drugs. Early laboratory diagnosis by isolation may be helpful in the medical management of these patients.
Viruses that may be recovered in cell culture include adenovirus, CMV, enterovirus, HSV, VZV, RSV, influenza virus, and parainfluenza virus. HSV and enterovirus are the most commonly recovered viruses.
A number of viruses are not routinely detected in cell culture. These include Epstein-Barr virus (EBV), rubella virus (must order serology), human papillomavirus (HPV), Norwalk or norovirus, and West Nile virus.
Not applicable
A positive result indicates that virus was present in the specimen submitted. Clinical correlation is necessary to determine the significance of the result.
Negative results may be seen in a number of situations including absence of viral disease, inability of the virus to grow in culture (examples of organisms not detected by culture include Epstein-Barr virus, rubella virus, human papilloma virus, norovirus and West Nile virus), and nonviable organisms submitted. Parainfluenza virus type 4 also may not be detected by viral culture.
Viral isolation and detection depends on the proper collection and transport of the specimen.
Some viruses (eg, cytomegalovirus) take up to 2 weeks to grow in viral cell culture. Molecular tests (ie, real-time PCR) should be used for rapid diagnosis.
1. Clinical and Laboratory Standards Institute (CLSI). Viral Culture. Proposed Guideline. 2005 CLSI document M41-P. CLSI, Wayne, PA
2. Ginocchio CC, Van Horn G, Harris PC: In Manual of Clinical Microbiology. Chapter 80: Reagents, stains, and cell culture: virology. 11th edition. Edited by J Versalovic, KC Carroll, et al. Washington, DC, ASM Press, 2015, pp 1422-1431
Results of viral cultures are reported from presence of typical cytopathic effect, and/or confirmation by immunofluorescence with specific antisera, or rapid PCR, depending on the specific virus.(Smith TF: Antibody-enhanced detection of viruses in cell cultures. In Manual of Clinical Laboratory Immunology. Fifth edition. Edited by NR Rose, EC de Macario, JD Folds, et al: Washington, DC, ASM Press, 1997, pp 618-624)
Monday through Sunday
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
87253-Additional Testing Virus Identification (if appropriate)
87254-Viral Smear, Shell Vial(if appropriate)
Test Id | Test Order Name |
Order LOINC Value
Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and results codes of this test. LOINC values are provided by the performing laboratory.
|
---|---|---|
VRID2 | Culture Referred for ID, Virus | 6584-7 |
Result Id | Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
---|---|---|
VRID2 | Culture Referred for ID, Virus | 6584-7 |