Useful For
Suggests clinical disorders or settings where the test may be helpful
Aiding in the diagnosis of active histoplasmosis using serum specimens
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
Special Instructions
Library of PDFs including pertinent information and forms related to the test
Method Name
A short description of the method used to perform the test
Complement Fixation (CF)/Immunodiffusion
NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Yes
Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test
Histoplasma Ab, S
Aliases
Lists additional common names for a test, as an aid in searching
fungal serology
histo
Histoplasma capsulatum
Histoplasma Complement Fixation
Histoplasmosis
Immunodiffusion Serology for Fungi
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
Specimen Type
Describes the specimen type validated for testing
Serum
Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 0.5 mL
Special Instructions
Library of PDFs including pertinent information and forms related to the test
Forms
Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory
Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected
Gross hemolysis | Reject |
Gross lipemia | Reject |
Specimen Type |
Temperature |
Time |
Special Container |
Serum |
Refrigerated (preferred) |
14 days |
|
|
Frozen |
14 days |
|
Useful For
Suggests clinical disorders or settings where the test may be helpful
Aiding in the diagnosis of active histoplasmosis using serum specimens
Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.
Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
MYCELIAL BY COMPLEMENT FIXATION (CF)
Negative (positives reported as titer)
YEAST BY CF
Negative (positives reported as titer)
ANTIBODY BY IMMUNODIFFUSION
Negative (positives reported as band present)
Interpretation
Provides information to assist in interpretation of the test results
Complement fixation (CF) titers of 1:32 or higher indicate active disease. A rising CF titer is associated with progressive infection.
Positive immunodiffusion test results supplement findings of the CF test. The simultaneous appearance of both H and M precipitin bands indicates active histoplasmosis. The M precipitin band alone indicates early or chronic disease or a recent histoplasmosis skin test.
Patients infected with Histoplasma capsulatum demonstrate a serum antibody with a rising titer within 6 weeks of infection. A rising titer is associated with progressive infection. Specific antibody persists for a few weeks to a year, regardless of clinical improvement.
Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Recent histoplasmosis skin tests must be avoided because the test causes a misleading rise in complement fixation titer, as well as an M precipitin band, in approximately 17% of patients having previous exposure to Histoplasma capsulatum.
Cross-reacting antibodies sometimes present interpretive problems in patients having blastomycosis or coccidioidomycosis.
Clinical Reference
Recommendations for in-depth reading of a clinical nature
1. Kaufman L, Kovacs JA, Reiss E: Clinical Immunomycology. In: Manual of Clinical and Laboratory Immunology. 5th ed. ASP Press, 1997
2. Deepe GS: Histoplasma capsulatum histoplasmosis. In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020:3162-3176
Special Instructions
Library of PDFs including pertinent information and forms related to the test
Method Description
Describes how the test is performed and provides a method-specific reference
Both immunodiffusion and complement fixation (CF) tests are used to detect antibodies to Histoplasma capsulatum. For immunodiffusion, the antigen used is a culture filtrate. Histoplasmin H and M precipitins can be identified by the assay. For the CF test, antigens are histoplasmin and a yeast form antigen of Histoplasma capsulatum; the latter is more sensitive.(Roberts GD: Fungi. In: Washington II JA, ed. Laboratory Procedures in Clinical Microbiology. 2nd ed. Springer-Verlag, 1985; Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020)
PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information
No
Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.
2 to 7 days
Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
14 days
Rochester
Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.
- Authorized users can sign in to Test Prices for detailed fee information.
- Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
- Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.
Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
Test Id |
Test Order Name |
Order LOINC Value
|
SHSTO |
Histoplasma Ab, S |
90227-0 |
Result Id |
Test Result Name |
Result LOINC Value
Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure.
|
15121 |
Histoplasma Mycelial |
20573-2 |
15122 |
Histoplasma Yeast |
20574-0 |
15123 |
Histoplasma Immunodiffusion |
90232-0 |
Test Setup Resources
Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.
Excel | Create a PDF
SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.
SI Normal Reports |
SI Abnormal Reports