Test Catalog

Test Id : DCTR

Direct Antiglobulin Test (Polyspecific), Blood

Useful For
Suggests clinical disorders or settings where the test may be helpful

Demonstrating in vivo coating of RBCs with IgG or the complement component C3d in the following settings:

-Autoimmune hemolytic anemia

-Hemolytic transfusion reactions

-Drug-induced hemolytic anemia

Reflex Tests
Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests.

Test Id Reporting Name Available Separately Always Performed
DATR Direct Antiglobulin Tst (Poly) No No
DC3TR Direct Antiglobulin Test (C3) No No
DIGTR Direct Antiglobulin Test (IgG) No No

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When the direct antigobulin tests is positive, the DCTR test will be interpreted as “Invalid – See addl testing” and the DATR test will be performed. When direct antiglobulin (DATR) is positive, then the DATR reflex tests, DAT complement 3 and DAT IgG, will be performed at an additional charge.

Method Name
A short description of the method used to perform the test

Hemagglutination

NY State Available
Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name
Lists a shorter or abbreviated version of the Published Name for a test

Direct Antiglobulin Test (Poly)

Aliases
Lists additional common names for a test, as an aid in searching

DCTR

Neonatal Blood Evaluation

Direct Coombs, B

Coombs, Direct, Blood

Direct Antiglobulin Test

Poly Specific Coombs

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When the direct antigobulin tests is positive, the DCTR test will be interpreted as “Invalid – See addl testing” and the DATR test will be performed. When direct antiglobulin (DATR) is positive, then the DATR reflex tests, DAT complement 3 and DAT IgG, will be performed at an additional charge.

Specimen Type
Describes the specimen type validated for testing

Whole Blood EDTA

Shipping Instructions

Specimen must arrive within 48 hours of draw.

Specimen Required
Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Container/Tube: Pink top (EDTA)

Specimen Volume: 6 mL

Specimen Minimum Volume
Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

3 mL

Neonates: EDTA Micro tube 0.5 mL

Reject Due To
Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis OK

Specimen Stability Information
Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient (preferred) 48 hours
Refrigerated 48 hours

Useful For
Suggests clinical disorders or settings where the test may be helpful

Demonstrating in vivo coating of RBCs with IgG or the complement component C3d in the following settings:

-Autoimmune hemolytic anemia

-Hemolytic transfusion reactions

-Drug-induced hemolytic anemia

Testing Algorithm
Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When the direct antigobulin tests is positive, the DCTR test will be interpreted as “Invalid – See addl testing” and the DATR test will be performed. When direct antiglobulin (DATR) is positive, then the DATR reflex tests, DAT complement 3 and DAT IgG, will be performed at an additional charge.

Clinical Information
Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

IgG antibody or complement components secondary to the action of IgM antibody may be present on the patient's own RBCs or on transfused RBCs.

Reference Values
Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Negative

If positive, test DATR will be performed.

Interpretation
Provides information to assist in interpretation of the test results

Negative: No IgG antibody or complement (C3d) detected on the surface of the red cell.

 

Positive: Test DATR will be ordered and performed.

Cautions
Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Autoimmune hemolytic anemia can occasionally occur with a negative direct antiglobulin test.

 

The direct antiglobulin test can be positive in some apparently healthy individuals.

Clinical Reference
Recommendations for in-depth reading of a clinical nature

AABB Technical Manual. 19th edition. Edited by MK Fung, AF Eder, SL Spitalnik, CM Westhoff. AABB, 2017

Method Description
Describes how the test is performed and provides a method-specific reference

Antihuman globulin reacts with red cell-bound immunoglobulins to produce agglutination.(AABB Technical Manual. 19th edition. Edited by MK Fung, AF Eder, SL Spitalnik, CM Westhoff. AABB, 2017)

PDF Report
Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) Performed
Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday, Sunday

Report Available
The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 2 days

Specimen Retention Time
Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location
Indicates the location of the laboratory that performs the test

Rochester

Fees
Several factors determine the fee charged to perform a test. Contact your U.S. or International Regional Manager for information about establishing a fee schedule or to learn more about resources to optimize test selection.

  • Authorized users can sign in to Test Prices for detailed fee information.
  • Clients without access to Test Prices can contact Customer Service 24 hours a day, seven days a week.
  • Prospective clients should contact their Regional Manager. For assistance, contact Customer Service.

Test Classification
Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR) product.

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information
Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Clinic Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

86880

Test Setup Resources

Setup Files
Test setup information contains test file definition details to support order and result interfacing between Mayo Clinic Laboratories and your Laboratory Information System.

Excel | Pdf

Sample Reports
Normal and Abnormal sample reports are provided as references for report appearance.

Normal Reports | Abnormal Reports

SI Sample Reports
International System (SI) of Unit reports are provided for a limited number of tests. These reports are intended for international account use and are only available through MayoLINK accounts that have been defined to receive them.

SI Normal Reports | SI Abnormal Reports