TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: FIBR    
Fibroblast Culture for Biochemical and Molecular Testing, Tissue

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This processing test is for culturing skin fibroblasts or other biopsies for biochemical or molecular genetic studies. No analysis or interpretation of results is performed.

 

When this test is ordered, cryopreservation will always be performed at an additional charge. However, for multiple assays on a patient utilizing the ordered fibroblast culture, only one culture is required regardless of the number of assays ordered.

 

Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested. To avoid delays, indicate testing to be performed and provide external lab paperwork for send out testing.

 

If viable cells are not obtained within 30 days, client will be notified.

 

Mycoplasma screening will be performed on all successfully grown fibroblast cultures. If Mycoplasma is detected, all pending fibroblast clinical testing will be cancelled, and the samples will be discarded.

Specimen Type Describes the specimen type validated for testing

Tissue

Ordering Guidance

This test is most useful for skin biopsy specimens. The specimen is cryopreserved for a minimum of 3 years.

 

This test is not appropriate for products of conception (POC) or chorionic villus sampling (CVS) specimens. If this test is ordered for either a POC or CVS specimen, the test will be canceled and CULFB / Fibroblast Culture for Genetic Testing, Tissue will be performed as the appropriate test.

Necessary Information

1. Include patient clinical history with request. This information will be used for any testing performed on the cultured cells.

2. Indicate the tests to be performed on the fibroblast culture cells. If send out testing is requested, the external lab paperwork must be submitted.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Submit only 1 of the following specimens:

 

Specimen Type: Cultured fibroblasts

Container/Tube: T-75 or T-25 flask

Specimen Volume: 1 Full T-75 flask or 2 full T-25 flasks

Specimen Stability Information: Ambient/Refrigerated

 

Specimen Type: Skin biopsy

Supplies: Fibroblast Biopsy Transport Media (T115)

Container/Tube: Sterile container with any standard cell culture media (eg, minimal essential media, RPMI 1640). The solution should be supplemented with 1% penicillin and streptomycin. Tubes of culture media can be supplied upon request (Eagle's minimum essential medium with 1% penicillin and streptomycin).

Specimen Volume: 4-mm punch

Specimen Stability Information: Refrigerated/Ambient

Special Instructions Library of PDFs including pertinent information and forms related to the test

Forms

Biochemical Genetics Patient Information (T602) in Special Instructions.

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Tissue in formalin or fixative preservative Reject

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
TissueVaries