Test Catalog

Test ID: AMSU    
Amylase, Timed Collection, Urine

Specimen Type Describes the specimen type validated for testing


Necessary Information

For any timed collection the volume and length of collection is required.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Supplies: Aliquot Tube, 5 mL (T465)

Container/Tube: Plastic, 5-mL tube (T465)

Specimen Volume: 5 mL

Collection Instructions:

1. Collect a 2-hour urine specimen.

2. The patient should have nothing by mouth except water between the hours of 6 p.m. and 10 a.m.

3. The bladder should be completely emptied at 8 a.m. This urine is discarded.

4. An adequate urine specimen (>100 mL) is ensured if the patient drinks 3 or more 8-ounce glasses of water. Half of this amount should be ingested between 7:30 a.m. and 8 a.m. The second half should be ingested at 8:30 a.m.

5. Collect all urine after 8 a.m. in container supplied.

6. The collection ends at 10 a.m. Include the 10 a.m. void in the collection container.

7. Measure and record the 2-hour volume.

8. Overlay urine with toluene (5 mL), and send aliquot. If no toluene is available, refrigerate specimen during collection, and send the aliquot specimen frozen.

9. Record the date and time (the exact start and completion times of the 2-hour collection) on the container label.


If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
UrineRefrigerated (preferred)7 days
 Frozen 7 days