TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: TBNGS    
Mycobacterium tuberculosis Complex, Molecular Detection of Drug Resistance Markers, Whole Genome Sequencing, Varies

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Whole genome sequencing (WGS) of Mycobacterium tuberculosis complex isolates is performed followed by evaluation of selected genes of interest for the presence of well-characterized, drug resistance-conferring variants.

 

Traditional broth-based, phenotypic drug resistance testing should also be performed, since not all genes associated with resistance within the M tuberculosis complex genome have been fully elucidated or are evaluated in this test. If traditional broth-based phenotypic drug resistance testing is desired, add TB1LN / Antimicrobial Susceptibility, Mycobacterium tuberculosis Complex, First Line; TB2LN / Susceptibility, Mycobacterium tuberculosis Complex, Second Line; and TBPZA / Susceptibility, Mycobacterium tuberculosis Complex, Pyrazinamide.

Specimen Type Describes the specimen type validated for testing

Varies

Additional Testing Requirements

If traditional broth-based phenotypic drug resistance testing is desired, add TB1LN / Antimicrobial Susceptibility, Mycobacterium tuberculosis Complex, First Line; TB2LN / Susceptibility, Mycobacterium tuberculosis Complex, Second Line; and TBPZA / Susceptibility, Mycobacterium tuberculosis Complex, Pyrazinamide.

Shipping Instructions

See Infectious Specimen Shipping Guidelines in Special Instructions

Necessary Information

1. Specimen source is required.

2. Organism identification is required; if not provided then organism identification will be reflexed to CTBID / Culture Referred for Identification, Mycobacterium and Nocardia with additional charges.

3. Method of identification is required (eg, nucleic acid hybridization probes, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), DNA sequencing, line probe assay).

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Supplies: Infectious Container, Large (T146)

Specimen Type: Mycobacterium tuberculosis complex isolate growing in pure culture. Isolates older than 5 weeks or not in a pure culture may require subculture for fresh, isolated growth so the turnaround time for results may be delayed.

Container/Tube: Middlebrook (7H10 or 7H11) medium slant; growth in broth medium (eg, Mycobacteria Growth Indicator Tube [7H9] broth) or on a Lowenstein-Jensen medium slant can be sent but turnaround time for results may be delayed because subculture to Middlebrook agar medium may be required. Organisms received in mixture may result in additional charges for isolation and identification.

Specimen Volume: Isolate with visible growth on solid media; if broth is sent, > or =3 mL of broth culture required.

Collection Instructions:

1. Organism must be in pure culture, actively growing. Do not submit mixed cultures.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.

Special Instructions Library of PDFs including pertinent information and forms related to the test

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Other Agar plate Mixed culture

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
VariesAmbient (preferred)7 days
 Refrigerated 7 days