Test Catalog

Test ID: REVE    
Erythrocytosis Evaluation, Whole Blood

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This is a consultative evaluation in which the case will be evaluated at Mayo Clinic Laboratories, the appropriate tests performed at an additional charge, and the results interpreted.


This profile evaluates for hereditary (congenital) causes of erythrocytosis. Symptoms should be long-standing or familial in nature. All cases will be tested for p50 (if shipping control is received) and hemoglobin variants (cation exchange HPLC, capillary electrophoresis and mass spectrometry) with an interpretative report. Additional testing is guided in a reflexive manner, and may include molecular testing of the HBA1/HBA2, HBB, EPOR, VHL, EGLN1(PHD2), EPAS1(HIF2a), and BPGM genes, among others, as appropriate. See Erythrocytosis Evaluation Testing Algorithm in Special Instructions. An information sheet relaying clinical history, erythropoietin (EPO) levels, and JAK2 result s, if known, allows more complete interpretation.


An additional consultative interpretation that summarizes all testing, will be provided after test completion to incorporate subsequent results into an overall evaluation if any of the following molecular tests are reflexed:

-ATHAL / Alpha-Globin Gene Analysis, Varies

-WASQR / Alpha Globin Gene Sequencing, Blood

-WBSQR / Beta-Globin Gene Sequencing, Blood

-WBDDR / Beta-Globin Cluster Locus Deletion/Duplication, Blood

-WGSQR / Gamma-Globin Full Gene Sequencing, Varies

-BPGMM / 2,3-Bisphosphoglycerate Mutase, Full Gene Sequencing Analysis, Varies

-HEMP / Hereditary Erythrocytosis Mutations, Whole Blood

-VHLE / VHL Gene, Erythrocytosis Mutation Analysis


The following algorithms are available in Special Instructions:

-Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation

-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation


See Benign Hematology Evaluation Comparison in Special Instructions.

Specimen Type Describes the specimen type validated for testing

WB Sodium Heparin
Whole Blood EDTA

Advisory Information

Polycythemia vera and acquired causes of erythrocytosis should be excluded before ordering this evaluation.

Shipping Instructions

All 3 specimens must arrive within 72 hours of collection.

Necessary Information

Include recent transfusion information.


Include most recent CBC results.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

A total of 3 specimens are required to perform this profile; all 3 specimens must arrive within 72 hours of collection:

-Whole blood EDTA for A2F, HBEL, MASS

-Whole blood sodium heparin for P50*

-Normal shipping control: whole blood sodium heparin for P50*

*Please note: If sodium heparin patient and control specimens are not received, the P50 test cannot be performed.



Container/Tube: Lavender top (EDTA) and green top (sodium heparin)

Specimen Volume:

EDTA: 5 mL

Sodium heparin: 4 mL

Collection Instructions:

1. Immediately refrigerate specimens after collection.

2. Send specimen in original tube. Do not aliquot.

3. Rubber band patient specimen and control vial together.


Normal Shipping Control:

Container/Tube: Green top (sodium heparin)

Specimen Volume: 4 mL

Collection Instructions:

1. Collect a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.

2. Label clearly on outermost label normal control.

3. Immediately refrigerate specimen after collection.

4. Send specimen in original tube. Do not aliquot.

5. Rubber band patient specimen and control vial together.

Special Instructions Library of PDFs including pertinent information and forms related to the test


1. New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available in Special Instructions:

-Informed Consent for Genetic Testing (T576)

-Informed Consent for Genetic Testing-Spanish (T826)

2. Metabolic Hematology Patient Information (T810) is available in Special Instructions.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

EDTA blood: 2.5 mL
Sodium heparin blood: 1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Gross hemolysis Reject

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
ControlRefrigerated72 hoursGREEN TOP/HEP
WB Sodium HeparinRefrigerated72 hoursGREEN TOP/HEP
Whole Blood EDTARefrigerated72 hours