Test Catalog

Test ID: TLPF    
T-Cell Lymphoma, FISH, Varies

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.


When this test and flow cytometry testing for leukemia/lymphoma are ordered concurrently, the flow cytometry result will be utilized to determine if sufficient clonal T-cells are available for FISH testing. If the result does not identify a sufficient clonal T-cell population, this FISH test order will be canceled and no charges will be incurred.


If FISH testing proceeds, probes will be performed based on the lymphoma subtype suspected/identified utilizing the table located in Clinical Information.


If the patient is being tracked for known abnormalities, indicate which probes should be used.


The following probe sets are available within the T-cell lymphoma FISH profile:

14q32.1 rearrangement, TCL1A

/i(7q)/ -7/7q-, D7S486/D7Z1

+8, D8Z2/MYC


If T-cell prolymphocytic leukemia/lymphoma (T-PLL) is indicated, TCL1A rearrangement is performed, with reflex to the TRAD FISH probe if TCL1A is negative,


See Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm in Special Instructions.

Specimen Type Describes the specimen type validated for testing


Advisory Information

This assay detects chromosome abnormalities observed in the blood or bone marrow of patients with T-cell lymphoma.

-For testing paraffin-embedded tissue samples from patients with T-cell lymphoma, see TLYM / T-Cell Lymphoma, FISH, Tissue.

-For patients with T-cell acute lymphoblastic leukemia/lymphoma, order TALLF / T-Cell Acute Lymphoblastic Leukemia [T-ALL], FISH, Varies.

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

1. Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

2. A pathology or flow cytometry report may be requested by the Genomics Laboratory to optimize testing and aid in interpretation of results.

Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testing

Submit only 1 of the following specimens:



Specimen Type: Bone marrow

Container/Tube: Green top (sodium heparin)

Specimen Volume: 1-2 mL

Collection Instructions: Invert several times to mix bone marrow.



Specimen Type: Blood

Container/Tube: Green top (sodium heparin)

Specimen Volume: 7-10 mL

Collection Instructions: Invert several times to mix blood.

Special Instructions Library of PDFs including pertinent information and forms related to the test


If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Specimen Minimum Volume Defines the amount of sample necessary to provide a clinically relevant result as determined by the Testing Laboratory

Blood: 2 mL
Bone Marrow: 1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

No specimen should be rejected.

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the performing laboratory, alternate acceptable temperatures are also included

Specimen TypeTemperatureTimeSpecial Container
VariesAmbient (preferred)