Test Catalog

Test ID: HERPV    
Herpes Simplex Virus 1 and 2, Qualitative PCR, Varies

Method Description Describes how the test is performed and provides a method-specific reference

The Luminex ARIES system utilizes PCR chemistry for the detection and differentiation of herpes simplex virus-1 (HSV-1) and HSV-2. In brief, primary specimen is added to the sample chamber of an ARIES HSV-1 and-2 assay cassette. The cassette is then placed into an ARIES system magazine. A magazine can hold up to 6 cassettes. The magazine is inserted into an ARIES system, which can process 2 magazines simultaneously. A barcode on top of the HSV-1 and -2 assay cassette is automatically scanned by the ARIES system, associating a preloaded ARIES HSV-1 and -2 assay protocol file with the cassette. The HSV-1 and -2 assay protocol file contains the necessary parameters to run the cassette, analyze data, and generate reports.


Once a run is started, the Sample Processing Control (SPC) is automatically added to the sample chamber of the cassette to control for sample lysis, recovery of extracted nucleic acid, detection of inhibitory substances, and confirmation of PCR reagent integrity. Sample and SPC lysis, as well as isolation and purification of nucleic acids, are automated within the ARIES system and the ARIES HSV 1 and 2 assay cassette. Purified nucleic acids are automatically transferred to the cassette's PCR tube that contains the lyophilized HSV-1 and -2 master mix for the PCR amplification step.(Binnicker MJ, Espy MJ, Duresko B, et al. Automated processing, extraction and detection of herpes simplex virus types 1 and 2: A comparative evaluation of three commercial platforms using clinical specimens. J Clin Virol 2017;89:30-33)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Saturday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 week

Performing Laboratory Location Indicates the location of the laboratory that performs the test