Test Catalog

Test ID: SCDT2    
Severe Combined Immunodeficiency Syndrome (SCID) Newborn Screening, Blood Spot

Method Description Describes how the test is performed and provides a method-specific reference

The droplet digital PCR (ddPCR) TREC screening assay detects severe combined immunodeficiency syndromes (SCID) by measuring absolute concentrations of T-cell receptor excision circles (TREC) and ribonuclease P (RNAseP, RPP30, a reference gene) in DBS by fluorescent droplet.(Vidal-Folch N, Milosevic D, Majumdar R, et al: A Droplet Digital PCR Method for Severe Combined Immunodeficiency Newborn Screening. J Mol Diagn 2017, Sep 19 (5): 755-765. doi: 10.1016/j.jmoldx.2017.05.011) An absolute TREC concentration is determined by applying a Poisson distribution calculation across the positive and negative droplets.(Pinheiro LB, Coleman VA, Hindson CM, et al: Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification. Anal Chem 2012, 84;1003-1011. doi: 10.1021/ac202578x. Epub 2011 Dec 21)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Sunday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

5 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

10 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Minimum of 1 year

Performing Laboratory Location Indicates the location of the laboratory that performs the test