Test Catalog

Test ID: ELYMI    
Lyme Disease European Immunoblot, Serum

Method Description Describes how the test is performed and provides a method-specific reference

The European Borrelia IgM and IgG immunoblots are based on an enzyme-immunoassay in a microarray format, carrying highly purified, specific native antigens from Borrelia afzelii (Pko strain) and Borrelia burgdorferi sensu stricto, as well as recombinant VlsE at defined position on a solid-phase nitrocellulose membrane in triplicate. The positions of these antigen "spots" are well defined and are reliably identifiable using customized software. Each microarray also has spots for a negative control, serum controls, conjugate controls, and 6 calibrators.


One microarray is fixed at the bottom of each well in a standard microtiter plate (MTP). For each test to be performed, the diluted patient serum is added to each microarray (note: the Borrelia IgM and IgG microarrays are in separate wells). If specific antibodies recognizing a Borrelia antigen are present, they will bind to the specific antigens on the microarray. After incubation the microarray is washed to remove unbound antibodies. Alkaline-phosphatase-antihuman IgG or antihuman IgM (conjugate) is then added to the well and incubated. If antibodies are present, the conjugate will bind to those respective antibodies, and, after a washing step to remove unbound conjugate, substrate solution is added. If the antibody/conjugate complex is present, the substrate will undergo precipitation and color change. After an incubation period, the reaction is stopped and the presence of precipitated substrate is visualized at specific locations on the microarray. The presence of a colored precipitation at various locations on the microarray is an indirect measurement of Borrelia specific antibodies in the patient specimen. Visualized spots from the reaction are compared for intensity with the integrated calibrator controls for evaluation. The IgM-analyte spots serve to detect antibodies against p41, p39, OspC, Osp17, and VlsE. The IgG-analyte spots serve to detect antibodies against p83, p58, p43, p39, p30, p21, OspC, DbpA/Osp17, p14, and VlsE.(Package insert: Borrelia ViraChip IgM and IgG Test kits. ViraMed; 07/2018)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Friday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 day

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test