Web: | mayocliniclabs.com |
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Email: | mcl@mayo.edu |
Telephone: | 800-533-1710 |
International: | +1 855-379-3115 |
Values are valid only on day of printing. |
Purified antigens are bonded to a microwell plate. Antibodies that are present in the patient samples attach themselves to the antigens and are determined during the second phase of the test by using enzyme-labelled antihuman antibodies (the conjugate). The enzyme converts the colorless substrate (urea peroxide/TMP) to a blue endproduct. The enzyme reaction is stopped by adding sulfuric acid. The colour of the mixture then switches from blue to yellow. A final measurement is carried out in a photometer at 450 nm using a reference wavelength of > or =620 nm.(Package insert: RIDASCREEN Echinococcus IgG. R-Biopharm AG: 09/2015)
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