Test Catalog

Test ID: VHSV    
Herpes Simplex Virus (HSV), Culture From Neonates, Varies

Method Description Describes how the test is performed and provides a method-specific reference

This test is intended for use in the identification and typing of herpes simplex virus (HSV) in cell culture. The test can detect and distinguish between HSV-1 and HSV-2 in shell-vial cultures (MRC-5 cells) before the development of cytopathic effect (Pre-CPE). This test uses monoclonal antibodies (DHI/Quidell), labeled with fluorescein isothiocyanate, which react specifically with HSV-1 and HSV-2. The testing process involves the inoculation of shell vials containing a monolayer of cells on a 12-mm circular cover slip at the base of the vial. Specimen is inoculated into the shell vial and infection of the cells is enhanced by low-speed centrifugation, which is thought to enhance viral infection of the cell monolayer. After incubation at 35 degrees C to 37 degrees C, monoclonal antibodies directed against immediate early antigens of replicating HSV are added, and viral-specific foci visualized by fluorescence microscopy.(Paya CV, Wold AD, Smith TF: Detection of cytomegalovirus infections in specimens other than urine by the shell vial assay and conventional tube cell cultures. J Clin Microbiol 1987;25:755-757)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

2 to 4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Until Reported

Performing Laboratory Location Indicates the location of the laboratory that performs the test