Test Catalog

Test ID: HSTQU    
Histoplasma Antigen, Quantitative Enzyme Immunoassay, Random, Urine

Method Description Describes how the test is performed and provides a method-specific reference

The IMMY Clarus Histoplasma GM Enzyme Immunoassay (EIA) is an immunoenzymatic, sandwich microplate assay which detects Histoplasma galactomannan in urine. Galactomannan is a polysaccharide found in the cell wall. Monoclonal anti-Histoplasma IgG antibodies bound to microwell plates are used as capture antibodies. Horseradish peroxidase (HRP) conjugated anti-Histoplasma monoclonal IgG antibodies are used as detection reagents. Urine specimens are run untreated. The samples are added to the microwells coated with the capture antibodies and incubated.


If the patient specimen contains Histoplasma galactomannan, those antigens will become bound to the capture antibodies on the microwells. After incubation, the microwells are washed to remove unbound patient material. HRP detection antibodies are added to the microwells. After a second incubation, the microwells are washed to remove any unbound HRP detection antibodies. If antigen is present in the patient sample, a blue color develops with the addition of 3,3’,5,5’ tetramethylbenzidine (TMB). The reaction is stopped by the addition of a stop solution, where a yellow color develops. The optical density (absorbance) is checked with a microplate reader at 450 nm and a reference wavelength of 620/630 nm.(Package insert: Clarus Histoplasma GM Enzyme Immunoassay. IMMY; Rev. 0 05/08/2019)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test