TEST CATALOG ORDERING & RESULTS SPECIMEN HANDLING CUSTOMER SERVICE EDUCATION & INSIGHTS
Test Catalog

Test ID: TCZ    
Tocilizumab Quantitation, Serum

Method Description Describes how the test is performed and provides a method-specific reference

This immunoassay is designed to determine tocilizumab (TOC) concentrations in human serum by means of electrochemiluminescence (ECL). The assay uses a "bridging" format in which TOC forms a link between biotin labeled anti-tocilizumab and Sulfo-Tag labeled anti-tocilizumab. During sample preparation, biotinylated and Sulfo-Tagged antibodies to tocilizumab are added and bind with drug that is present in the sample. After the incubation with the labeled antibodies, the calibrators, controls, and samples are added to a streptavidin plate that has been blocked with a solution of bovine serum albumin. After an incubation period, the plate is washed and read buffer is added. Immediately after the addition of read buffer, the plate is analyzed. The read buffer provides an appropriate chemical environment for ECL when a voltage is applied to the electrodes on the plate. This voltage causes bound Sulfo-Tagged anti-tocilizumab to emit measureable light. The intensity of emitted light is measured and is correlated to a set of standards with known concentrations of TOC by means of a 4-point logistics curve fitting method.(Unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday, Thursday

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Clinic Laboratories until the release of the test result

5 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test

Rochester