Test Catalog

Test ID: SARS2    
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA, Varies

Method Description Describes how the test is performed and provides a method-specific reference

The Abbott RealTime SARS-CoV-2 assay is performed using the FDA-approved, semi-automated Abbott m2000 system, which comprises the Abbott m2000sp sample preparation instrument and the Abbott m2000rt real-time PCR thermocycler. The Abbott mSample Preparation System kit is used on the Abbott m2000sp instrument, based on magnetic microparticle technology, to extract and purify viral RNA from clinical upper respiratory tract swab specimens and assay controls (negative and positive) placed in 96-well microtiter plate format. An internal control is also added to the extraction reagents and carried through the entire process in each specimen to ensure adequate extraction and subsequent target amplification have occurred free of inhibitory substances.


The PCR assay mastermix contains 3 sets of primers and TaqMan probes targeting sequences in the RdRp and N genomic regions of SARS-CoV-2 and the internal control armored RNA. Amplification and detection of target sequences are performed on the Abbott m2000rt thermocycler. Clinical samples containing SARS-CoV-2 would yield detectable signals for the RdRp and N sequences.(FAM-labeled fluorescent probes) as well as the internal control gene sequence (VIC-labeled fluorescent probe)(Package insert: Abbott RealTime SARS-CoV-2. Abbott Molecular, Inc; Doc. 51-608445/R1, 03/2020)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) Performed Outlines the days the test is performed. This field reflects the day that the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time before the test is performed. Some tests are listed as continuously performed, which means that assays are performed multiple times during the day.

Monday through Sunday

Report Available The interval of time (receipt of sample at Mayo Clinic Laboratories to results available) taking into account standard setup days and weekends. The first day is the time that it typically takes for a result to be available. The last day is the time it might take, accounting for any necessary repeated testing.

Same day/1 to 3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

5 days

Performing Laboratory Location Indicates the location of the laboratory that performs the test